A review on forensic analysis of bio fluids (blood, semen, vaginal fluid, menstrual blood, urine, saliva): Spectroscopic and non-spectroscopic technique

Abstract

The accurate detection, identification, and analysis of biofluids at crime scenes play a critical role in forensic investigations. Various biofluids, such as blood, semen, vaginal fluid, menstrual blood, urine, and saliva, can be crucial evidence. In a murder case involving a knife attack, for instance, bloodstains from both the victim and perpetrator might be present. Sexual assault cases often involve the analysis of semen and vaginal secretions. Biofluid analysis employs a two-tiered approach: presumptive tests for initial identification and confirmatory tests for definitive analysis. This review article focuses on six key biofluids and their forensic significance. In this review, we comprehensively explore the relevant analytical techniques, including non-spectroscopic methods like immunoassays, spot tests, and cytokine profiling, alongside spectroscopic techniques such as Infrared (IR) spectroscopy, Mass Spectrometry (MS), and Raman Spectroscopy (RS).