Protective role of vitamin B6 on some histological changes in the liver and kidneys and measure some indicators of oxidation balance in male rats.

Abstract

This study aimed to evaluate the therapeutic effects of B6 in rats experimentally intoxicated by benzopyrene. Twenty-eight Male Sprague Dawley (white Swiss) rats weighing 170-210 g and 3-4 months old were utilized in this examination. Rats were divided into 4 control groups (G1), B[a]P 2 pmol/μL (G2), B6 only once per 2 days for a full month at 1000 mcg (15 dose per month) (G3), B6 + B[a]P (G4). The results showed an increase in the level of MDA and a significant decrease in the level of GSH in the second group compared to the negative control group, while no significant differences appeared in the third group, while a significant decrease in the level of MDA and a significant increase in the level of GSH were observed in the fourth group when compared with The second group. Hepatic and renal tissues were taken for histopathological study. The results showed that liver and kidney of G1 and G3 exhibit normal architecture. Liver of G2 revealed blood congestion in certain sinusoids and atrophied hepatocytes, there was also hyperplasia of Kupffer cells in the pockets of blood sinusoids, while renal tissues showed inflammatory cell infiltration, mesangial cell hyperplasia, and blood vessel congestion and bleeding. In contrast liver and kidney tissues in G4 showed mild lesion after B6 treatment. In conclusion, Pyredoxin (B6) can alleviate the hepatic and renal tissues damaged caused by benzopyrene.