A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-21 DOI:10.1016/j.talanta.2024.127433

Shuiyuan Liang, Sha Chen, Zhenlin Xu, Jiahong Chen, Hongtao Lei, Tian Guan

{"title":"A smartphone-based dual-modal lateral flow immunochromatographic assay for multiplex detection of illegal stimulant laxatives in slimming foods.","authors":"Shuiyuan Liang, Sha Chen, Zhenlin Xu, Jiahong Chen, Hongtao Lei, Tian Guan","doi":"10.1016/j.talanta.2024.127433","DOIUrl":null,"url":null,"abstract":"<p><p>Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs). Due to the enhanced local surface plasmon resonance and absorption intensity of our unique cAuNPs, this LFIA can simultaneously detect bisacodyl, bis-(p-hydroxyphenyl)-pyridyl-2-methane and sodium picosulfate in slimming food as low as 0.014-0.308 ng/mL by a smartphone-based photothermal device or 1.4-10 ng/mL by naked eyes. Compared with traditional AuNPs-LFIA, it demonstrated 8.48-10.27 times higher sensitivity in photothermal mode. Moreover, in the recovery test and blind sample analysis, this dual-modal LFIA exhibited reasonable recoveries (85.04 %-119.18 %) and high correlation with authorized LC-MS/MS method, suggesting high accuracy and applicability. This work offers a highly sensitive, accurate and convenient way for the on-site determination of stimulant laxatives, which can also be employed to detect other food hazards, environmental pollutants, or clinical analytes within a few minutes.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"285 ","pages":"127433"},"PeriodicalIF":5.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2024.127433","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/21 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Stimulant laxatives (especially bisacodyl and sodium picosulfate) are frequently found to be adulterated into slimming foods, causing health-threatening effects to consumers. Sensitive, accurate, easy-to-operate and portable multiplex analytical techniques are still desired for the rapid screening of stimulant laxatives in slimming foods. In this work, a highly sensitive dual-modal colorimetric/photothermal lateral flow immunochromatographic assay (LFIA) was established based on facilely prepared concentrated gold nanoparticles (cAuNPs). Due to the enhanced local surface plasmon resonance and absorption intensity of our unique cAuNPs, this LFIA can simultaneously detect bisacodyl, bis-(p-hydroxyphenyl)-pyridyl-2-methane and sodium picosulfate in slimming food as low as 0.014-0.308 ng/mL by a smartphone-based photothermal device or 1.4-10 ng/mL by naked eyes. Compared with traditional AuNPs-LFIA, it demonstrated 8.48-10.27 times higher sensitivity in photothermal mode. Moreover, in the recovery test and blind sample analysis, this dual-modal LFIA exhibited reasonable recoveries (85.04 %-119.18 %) and high correlation with authorized LC-MS/MS method, suggesting high accuracy and applicability. This work offers a highly sensitive, accurate and convenient way for the on-site determination of stimulant laxatives, which can also be employed to detect other food hazards, environmental pollutants, or clinical analytes within a few minutes.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

基于智能手机的双模态横向流动免疫层析检测减肥食品中非法兴奋剂泻药的多重检测。

刺激性泻药（特别是比沙酰和皮硫酸钠）经常被掺入减肥食品中，对消费者的健康造成威胁。灵敏，准确，易于操作和便携的多重分析技术仍然需要快速筛选减肥食品中的兴奋剂泻药。在这项工作中，建立了一种高灵敏度的双模态比色/光热侧流免疫层析（LFIA）方法，该方法基于制备的浓缩金纳米颗粒（cAuNPs）。由于我们独特的cAuNPs的局部表面等离子体共振和吸收强度增强，该LFIA可以同时检测减肥食品中低至0.014-0.308 ng/mL的双-（对羟基苯基）-吡啶-2-甲烷和pico硫酸钠，通过智能手机光热装置或1.4-10 ng/mL的裸眼检测。与传统的AuNPs-LFIA相比，它在光热模式下的灵敏度提高了8.48-10.27倍。在回收率测试和盲样分析中，该双模态LFIA具有合理的回收率（85.04% ~ 119.18%），且与授权的LC-MS/MS方法具有较高的相关性，具有较高的准确性和适用性。本工作为刺激性泻药的现场检测提供了一种高度灵敏、准确和方便的方法，也可用于其他食品危害、环境污染物或临床分析物的检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.