Manganese porphyrin wrapped DNA dendrimer as a universal chemiluminescence tag for ultrasensitive and multiplex assay of mycotoxins.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-18 DOI:10.1016/j.talanta.2024.127418

Qiushuang Tian, Haoyan Zhou, Ruike Wang, Ling Xu, Jun Chen, Chen Zong

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Abstract

A manganese porphyrin wrapped DNA dendrimer (Mn-DD) was developed through enzyme-free DNA self-assembly and simple and mild groove binding of porphyrin. The Mn-DD not only possessed plenty of manganese porphyrin to amplify the chemiluminescence (CL) signal, but also can be modified with diverse groups via DNA hybridization. Combined with an immunosensor array, Mn-DD can be utilized for CL immunoassay of multiple mycotoxins as a universal tag. Under optimal conditions, Mn-DD-based CL imaging immunoassay of aflatoxin B₁ (AFB₁), ochratoxin A (OTA), and zearalenone (ZEN) exhibited broad linear ranges over 4 orders of magnitude and detection limits as low as 0.87, 0.75, and 0.79 pg mL^-1, respectively. It was also utilized in the examination of real coix seed samples, yielding reliable results. High sensitivity, as well as simple operation, low reagent dosage, acceptable accuracy and stability showed the tag and the approach broad application prospects in quality control of food and medicine.

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锰卟啉包裹DNA树状大分子作为一种通用化学发光标记，用于真菌毒素的超灵敏和多重检测。

通过无酶DNA自组装和简单温和的卟啉槽结合，制备了锰卟啉包裹DNA树突状结构（Mn-DD）。Mn-DD不仅含有大量的锰卟啉来放大化学发光信号，而且可以通过DNA杂交修饰成不同的基团。结合免疫传感器阵列，Mn-DD可作为通用标签用于多种真菌毒素的CL免疫分析。在最佳条件下，基于mn - dd的黄曲霉毒素B1 （AFB1）、赭曲霉毒素A （OTA）和玉米赤霉烯酮（ZEN）的CL成像免疫分析在4个数量级以上呈宽线性范围，检出限分别低至0.87、0.75和0.79 pg mL-1。它也被用于检测真正的薏苡仁样品，产生可靠的结果。该方法灵敏度高，操作简单，试剂用量少，准确度和稳定性好，在食品和药品质量控制中具有广阔的应用前景。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.