Haitham Saleh, Abdelfattah Badr, Ehab M Zayed, Elham R S Soliman
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引用次数: 0
Abstract
Pearl millet {Pennisetum glaucum (L.) R. Br} is a C4 panicoid cereal millet crop grown in arid and semi-arid regions in Africa and Asia for food and fodder. This study involves the evaluation of the genetic diversity of 28 worldwide germplasm collection of pearl millet by genetic markers polymorphism and drought tolerance indices. The genetic diversity was expressed by 51 alleles of 9 ISSR markers that showed 96.43% total polymorphism and 11.76 alleles per marker. Cluster analysis of ISSR markers polymorphism divided the 28 genotypes into four clusters partially in agreement with their origin. The application of drought stress simulated by 20% PEG6000 treatment, retarded the germination percentage, and reduced shoot and root length, seedling fresh and dry weights. Drought tolerance indices (DTIs) were calculated based on the response of the seedling traits under drought stress compared to the control seedlings. ANOVA revealed statistically significant variation among the genotypes (P ≤ 0.05), except for seedling fresh weight (P = 0.17 > 0.05) under control conditions and seedling dry weight (P = 0.99 > 0.05) under drought conditions. Genotypes having higher DTIs for three traits are regarded drought resistant, i.e., those from India, Ethiopia, Pakistan, and Nigeria. The calculated heritability values indicated that seedlings dry weight is the least trait affected by drought stress whereas root length is the most influenced trait. Hierarchical clustering, based on the DTI values, also grouped the genotypes partially concomitant to their origin. The correlation analysis demonstrated a modest positive correlation between shoot length and root length. A low correlation of r ≤ 0.12 was observed between the morphological DTI matrix and the genetic matrix. Nevertheless, high levels of genetic diversity were identified among the examined genotypes that may face genetic erosion by climatic constraints, and a high potential for creating agronomically superior cultivars by crossing widely divergent genotypes.
期刊介绍：
Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses.
Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication.
Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses.
Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods.
Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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