{"title":"Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants","authors":"Nobuo Arai , Yukino Tamamura-Andoh , Taketoshi Iwata , Ayako Watanabe-Yanai , Masahiro Kusumoto","doi":"10.1016/j.mimet.2024.107082","DOIUrl":null,"url":null,"abstract":"<div><div>We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for <em>Salmonella enterica</em> subsp. <em>enterica</em> serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.</div></div>","PeriodicalId":16409,"journal":{"name":"Journal of microbiological methods","volume":"228 ","pages":"Article 107082"},"PeriodicalIF":1.7000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microbiological methods","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0167701224001945","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.
期刊介绍：
The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach.
All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
