Qiu’s Cervical Prescription inhibit the invasion and growth of cervical cancer through LncRNA ATB/miR-126 pathway

IF 2.9 4区医学 Q2 PATHOLOGY Pathology, research and practice Pub Date : 2025-02-01 DOI:10.1016/j.prp.2024.155784

Yingping Zhu , Yang Lv , Haili Yao , Zhilei Chen , Wenjuan Yang , Chuntao Tian , Weiyi Yang , Mingyang Li , Qingge Jia , Liangping Wang

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Abstract

Background

Cervical cancer (CC) is one of the most deadly cancers in women, its current treatments still result in poor outcomes and developing the novel targets and therapeutic strategies are urgently needed. Qiu’s Cervical Prescription (QCP) is one of the traditional Chinese medicines used in the treatment of cervical cancer in China. Although its curative effect is remarkable, the internal mechanism of its treatment is still poorly understood. Recent studies have shown that LncRNA ATB might be used as a new proliferation marker for cancer diagnosis and prognosis. This study aimed to investigate the possible mechanism of action of QCP in the treatment of cervical cancer.

Methods

The functional assays of migration and invasion in vitro using transwell assays and wound healing assays was performed to confirm the pro-carcinogenic effect of LncRNA ATB, and the changes of migration and invasion of HeLa cells were observed after treatment with QCP containing drug serum. The changes in tumor volume, general condition of transplanted tumor-bearing mice and expression of LncRNA ATB pathway-related proteins were detected by qPCR, Western blotting and HE staining after treatment with the QCP.

Results

We induced LncRNA ATB knockdown and overexpression in cervical cancer cell lines and detected the biological behavior changes in vitro. Furthermore, we established murine models using stable LncRNA ATB-shRNA HeLa cells or overexpression LncRNA ATB cells or normal Hela cells with QCP to evaluate how suppression of LncRNA ATB affects tumor growth.

Conclusion

We showed that potential mechanism of QCP in the treatment of cervical cancer may be through inhibition of the LncRNA ATB/miR-126/TGFβ1 signaling axis. In conclusion, QCP may be a promising approach for the treatment of CC.

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Qiu’s Cervical方通过LncRNA ATB/miR-126通路抑制宫颈癌的侵袭和生长。

背景：宫颈癌（CC）是女性最致命的癌症之一，目前的治疗效果仍然很差，迫切需要开发新的靶点和治疗策略。邱氏子宫颈癌方（QCP）是中国治疗宫颈癌的传统中药之一。虽然其疗效显著，但其治疗的内在机制尚不清楚。近年来的研究表明，LncRNA ATB可能作为一种新的肿瘤诊断和预后增殖标志物。本研究旨在探讨芪多糖治疗宫颈癌的可能作用机制。方法：采用transwell法和创面愈合法对LncRNA ATB进行体外迁移和侵袭功能测定，证实LncRNA ATB的促癌作用，并观察含药血清QCP对HeLa细胞迁移和侵袭的影响。应用qPCR、Western blotting和HE染色检测移植荷瘤小鼠肿瘤体积、一般情况及LncRNA ATB通路相关蛋白表达的变化。结果：在宫颈癌细胞株中诱导LncRNA ATB敲低和过表达，并在体外检测其生物学行为变化。此外，我们使用稳定的LncRNA ATB- shrna HeLa细胞或过表达LncRNA ATB细胞或正常HeLa细胞与QCP建立小鼠模型，以评估LncRNA ATB抑制如何影响肿瘤生长。结论：我们发现QCP治疗宫颈癌的潜在机制可能是通过抑制LncRNA ATB/miR-126/ tgf - β1信号轴。综上所述，QCP可能是一种很有前途的治疗CC的方法。

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来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.