Haozi Lu, Junhua Wang, Ziyi Wu, Meiqing Yang, Wei Zhou, Ye Li, Huimin Li, Yang Zhang, Jifei Yang, Gang Yu, Song Liu
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引用次数: 0
Abstract
Sweat lactate levels are closely related to an individual's physiological state and serve as critical indicators for assessing exercise intensity, muscle fatigue, and certain pathological conditions. Screen-printed electrodes (SPEs) offer a promising avenue for the development of low-cost, high-performance wearable devices for electrochemical sweat analysis. The material composition of SPEs significantly impacts their detection sensitivity and stability. In this study, we designed a screen-printed carbon electrode (SPCE) modified with Ti3C2Tx Polydopamine (PDA), and silver nanoparticles (AgNPs) (Ti3C2Tx-PDA-AgNPs) for lactate detection in sweat. The accordion-like structure of Ti3C2Tx provides a large specific surface area and exceptional electrical conductivity. PDA, acting as both a reducing agent and binder, supports the in-situ formation of AgNPs on the Ti3C2Tx nanosheets. These AgNPs prevent the restacking of Ti3C2Tx layers, further improving conductivity. The sensor exhibited sensitivities of 0.145 μA mM-1, with limit of detection (LOD) of 0.181 mM (S/N = 3) in phosphate-buffered saline (PBS), meeting the requirements for for sweat lactate detection. The sensor was integrated into a wearable micro-electrochemical platform paired with a custom Android application for real-time sweat analysis. Testing on human sweat demonstrated the platform's potential for practical fitness monitoring and healthcare diagnostics applications.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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