Modeling, synthesis and in vitro testing of peptides based on unusual amino acids as potential antibacterial agents.

Q3 Biochemistry, Genetics and Molecular Biology Biomeditsinskaya khimiya Pub Date : 2024-12-01 DOI:10.18097/PBMC20247006413

A S Sargsyan, L T Karapetyan, A V Mkhitaryan, L A Stepanyan, T H Sargsyan, Yu M Danghyan, A V Sargsyan, G G Oganezova, N A Hovhannisyan

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Abstract

Currently non-protein amino acids and synthetic peptides are widely used as blocks in drug design. Many proteases are of great interest for pharmacology due to their key role in various pathologies. Bacterial collagenase (EC 3.4.24.3) is quite an attractive target for drug development as the inhibitors of bacterial collagenolytic protease may stop propagation of diseases caused by infections. The interaction of peptides containing unusual amino acids with Clostridium histolyticum collagenase has been evaluated by molecular docking followed by the measurement of enzyme inhibition by selected compounds. According to the docking analysis, 4 compounds were selected and synthesized for further research. Measurement of enzyme activity revealed that all tested compounds inhibited collagenase activity with IC50 values ranging within 1.45-2.08 μM. The antibacterial activity of synthesized compounds against some resistant strains was characterized by MICs values ranging within 4.6-9.2 μg/ml.

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基于特殊氨基酸的潜在抗菌肽的建模、合成和体外测试。

目前，非蛋白质氨基酸和合成肽在药物设计中被广泛用作阻滞剂。许多蛋白酶由于在各种病理中起关键作用而引起药理学的极大兴趣。细菌胶原酶（EC 3.4.24.3）是一个很有吸引力的药物开发靶点，因为细菌溶胶原蛋白酶的抑制剂可以阻止感染引起的疾病的传播。含有异常氨基酸的肽与溶组织梭菌胶原酶的相互作用已通过分子对接进行了评估，随后测量了选定化合物对酶的抑制作用。根据对接分析，选择并合成了4个化合物进行进一步研究。酶活性测定表明，所有化合物对胶原酶的抑制作用IC50值在1.45 ~ 2.08 μM之间。合成的化合物对部分耐药菌株的抑菌活性在4.6 ~ 9.2 μg/ml之间。

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).