Crystallization and preliminary X-ray crystallographic studies of AfeH from Acimetobacter sp. DL-2

IF 1.1 4区 生物学 Q4 BIOCHEMICAL RESEARCH METHODS Acta crystallographica. Section F, Structural biology communications Pub Date : 2025-01-03 DOI:10.1107/S2053230X24012275
Huiling Tang, XiaoRan Zhang, Yajuan Duan, Yan Huang
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Abstract

Fenoxaprop-P-ethyl (FE) is widely applied as a post-emergence aryloxyphenoxy propionate (AOPP) herbicide in agriculture. A novel FE hydrolase esterase from Acinetobacter sp. DL-2 (AfeH) was identified which belongs to the family IV carboxylesterases and shows less than 30% identity to other reported homologues with known structure. In order to understand the catalytic mechanism, recombinant AfeH was prepared in Escherichia coli and crystallized using the sitting-drop vapor-diffusion method. X-ray diffraction data were collected to 1.9 Å resolution. The crystal belonged to space group P1211, with unit-cell parameters a = 84.27, b = 46.74, c = 258.68 Å. The Matthews coefficient (VM) was calculated to be 2.43 Å3 Da−1, which corresponds to a solvent content of 49.4%, suggesting the presence of three monomers in the crystallographic asymmetric unit. The crystal was assessed to be suitable for further structural determination, which is currently in progress.

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acmetobacter sp. DL-2中AfeH的结晶及x射线晶体学初步研究。
Fenoxaprop-P-ethyl (FE)作为一种涌现后丙酸芳氧苯氧基(AOPP)除草剂被广泛应用于农业。从不动杆菌DL-2 (Acinetobacter sp. DL-2, AfeH)中鉴定出一种新的FE水解酶酯酶,该酶属于IV家族羧酸酯酶,与其他已知结构的同源物的同源性低于30%。为了了解催化机理,在大肠杆菌中制备了重组AfeH,并采用坐滴气相扩散法结晶。x射线衍射数据采集至1.9 Å分辨率。晶体属于空间群P1211,晶胞参数a = 84.27, b = 46.74, c = 258.68 Å。计算出Matthews系数(VM)为2.43 Å3 Da-1,对应溶剂含量为49.4%,表明在结晶不对称单元中存在三种单体。该晶体被评估为适合进一步的结构测定,目前正在进行中。
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来源期刊
Acta crystallographica. Section F, Structural biology communications
Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY
CiteScore
1.90
自引率
0.00%
发文量
95
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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