A multi-valent polymyxin-based fluorescent probe for the detection of Gram-negative infections†

IF 6.1 3区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS Journal of Materials Chemistry B Pub Date : 2024-12-17 DOI:10.1039/D4TB01786B
Richa Sharma, Maria Rodriguez-Rios, James Crossland, Maulida Septiyana, Alicia Megia-Fernandez, Maxime Klausen and Mark Bradley
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Abstract

A multi-branched fluorogenic probe for the rapid and specific detection of Gram-negative bacteria is reported. Three Gram-negative-targeting azido-modified polymyxins were clicked onto a trivalent scaffold functionalised with the environmental green-emitting fluorophore 7-nitrobenz-2-oxa-1,3-diazole. The probe allowed wash-free detection of target bacteria with increased sensitivity and lower limits of detection compared to monovalent probes.

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一种检测革兰氏阴性感染的多价多粘菌素荧光探针。
报道了一种快速特异检测革兰氏阴性菌的多支荧光探针。三个革兰氏阴性靶向叠氮修饰的多粘菌素被点击到一个三价支架上,该支架与环保绿色荧光基团7-硝基苯-2-氧杂-1,3-二唑功能化。与单价探针相比,该探针允许无洗涤检测目标细菌,具有更高的灵敏度和更低的检测限。
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来源期刊
Journal of Materials Chemistry B
Journal of Materials Chemistry B MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
11.50
自引率
4.30%
发文量
866
期刊介绍: Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive: Antifouling coatings Biocompatible materials Bioelectronics Bioimaging Biomimetics Biomineralisation Bionics Biosensors Diagnostics Drug delivery Gene delivery Immunobiology Nanomedicine Regenerative medicine & Tissue engineering Scaffolds Soft robotics Stem cells Therapeutic devices
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