Characterization of the differences in lipid profiles and volatile compounds of adipose stem cells adipogenic differentiation and adipocytic transdifferentiation of muscle stem cells from large yellow croakers based on UPLC-MS/MS and GC-IMS

Abstract

Lipids contribute significantly to the flavor of cell-cultured fish meat as precursor components of flavor compounds. Here, we initially reported the differences in lipid metabolite profiles and volatile compounds between adipogenic differentiation of adipose stem cells (ASCs) and adipocytic transdifferentiation of muscle stem cells (MSCs) from large yellow croakers. A total of 2106 lipid metabolites were identified by UPLC-MS/MS. Compared with the MSCs group, 1263 differentially expressed lipid metabolites were found in the ASCs group, with 1026 up-regulated lipid metabolites and 237 down-regulated ones. The main lipid differences were fatty acyls (FAs) and glycerolipids (GL). GC-IMS identified a total of 47 volatile compounds. Volatile compounds such as ethyl laurate, nonanal, and 2,3-pentanedione were significantly higher in the ASCs group than in the MSCs group. GC-IMS results indicated that the ASCs group was more flavorful. This study provides a theoretical basis for developing cell-cultured fish meat with a familiar flavor.