Bovine Serum Albumin-Capped Fluorescent Copper Nanocluster Incorporated with 2D-Molybdenum Disulfide Nanosheets as a FRET-Based Immune Probe for the "Turn-On" Detection of cTnT.

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2025-01-20 Epub Date: 2024-12-27 DOI:10.1021/acsabm.4c01670

Ali Ibrahim Shkhair, Anju S Madanan, Susan Varghese, Merin K Abraham, Geneva Indongo, Greeshma Rajeevan, Arathy B K Kala, Sara Muneer Abbas, Sony George

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Abstract

Cardiovascular disease is the primary cause of mortality worldwide, as stated by the World Health Organization. We utilized the red fluorescence emitted by copper nanoclusters (CuNCs) to detect cardiac Troponin T (cTnT). We designed a fluorescent probe to detect cTnT using an on-off-on technique. The cTnT antibody was coupled on the surface of a bovine serum albumin-capped CuNC (Ab-BSA©CuNC) MoS₂ nanosheet, efficiently quenching the luminescence of Ab-BSA©CuNCs due to their high affinity and binding interaction by establishing an Ab-BSA©CuNC/MoS₂ platform. The luminescence of Ab-BSA©CuNCs was restored due to the antigen-antibody interaction. With a detection limit of 9.4 pg/mL, a linear relationship between the luminescence intensity concentrations of cTnT was observed in the 0.161-1.57 ng/mL ranges. Additionally, the efficacy of the developed technique to measure cTnT in blood serum samples was evaluated, and it exhibited a good recovery percentage.

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牛血清白蛋白覆盖的荧光铜纳米簇结合2d -二硫化钼纳米片作为基于fret的免疫探针，用于cTnT的“开启”检测。

世界卫生组织指出，心血管疾病是全世界死亡的主要原因。我们利用铜纳米簇（cucs）发出的红色荧光检测心肌肌钙蛋白T （cTnT）。我们设计了一种荧光探针，使用开关技术检测cTnT。将cTnT抗体偶联在牛血清白蛋白覆盖的CuNC (Ab-BSA©CuNC) MoS2纳米片表面，通过建立Ab-BSA©CuNC/MoS2平台，有效地猝灭了Ab-BSA©CuNC的高亲和力和结合相互作用。由于抗原-抗体相互作用，Ab-BSA©CuNCs的发光恢复。检测限为9.4 pg/mL，在0.161 ~ 1.57 ng/mL范围内，cTnT发光强度浓度呈线性关系。此外，对所开发的技术在血清样品中检测cTnT的有效性进行了评估，并显示出良好的回收率。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.