Green and sustainable production of isofraxidin from Acanthopanax senticosus with cellulose-based immobilized probiotics.

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2025-03-01 Epub Date: 2024-12-23 DOI:10.1016/j.enzmictec.2024.110574

Shuang Jin, Hongyao Cai, Cailiang Peng, Yupeng Cheng, Yubin Ren, Weili Liu, Yujie Fu, Chen Lv

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Abstract

This study utilizes deep eutectic solvent (DES)-assisted enhancement of cellulose-based immobilized probiotics for efficient biotransformation of isofraxidin from Acanthopanax senticosus. Among seven probiotic strains tested, Lactiplantibacillus plantarum CICC 20767 exhibited the best catalytic activity. We explored the effects of 12 different DESs with varying hydrogen bond donors on the conversion capacity of the immobilized probiotics, with the highest efficiency observed using ChCl/EG (4.0 wt %). The optimized process, with a solid-to-liquid ratio of 1:5 (g/mL), a temperature of 35.6 °C, a reaction time of 4 d, and a pH of 6.9, resulted in a 5.53-folds increase in isofraxidin yield, reaching 0.4034 mg/g, compared to the untreated sample (0.0729 mg/g). The immobilized probiotics retained excellent catalytic activity after 12 cycles of use, demonstrating their stability and potential for large-scale, green production of isofraxidin. This study presents a valuable method for industrial isofraxidin production and highlights the broad potential of this environmentally friendly bioconversion process in the pharmaceutical industry.

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纤维素基固定化益生菌绿色可持续生产刺五加异黄酮。

本研究利用深度共熔溶剂（DES）辅助强化纤维素基固定化益生菌，对刺五加中异黄皮苷进行高效生物转化。7株益生菌中，植物乳杆菌CICC 20767的催化活性最好。我们探索了12种不同氢键供体的DESs对固定化益生菌转化能力的影响，其中使用ChCl/EG（4.0 wt %）的效率最高。在料液比为1:5 （g/mL）、温度为35.6 ℃、反应时间为4 d、pH为6.9的条件下，异黄皮苷的得率达到0.4034 mg/g，是未处理样品（0.0729 mg/g）的5.53倍。在12个循环使用后，固定化益生菌仍保持了良好的催化活性，证明了其稳定性和大规模绿色生产异黄菌素的潜力。本研究提出了一种有价值的工业异黄酮生产方法，并强调了这种环境友好型生物转化工艺在制药工业中的广泛潜力。

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公司名称

产品信息

阿拉丁

Acetonitrile

来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.