Radiocarbon Dating of Gallstones for Timeline of Formation, Dormancy Analysis, and Correlation with Chemical/Microbial Composition: A Pilot Study on Gallstone Geobiology.
{"title":"Radiocarbon Dating of Gallstones for Timeline of Formation, Dormancy Analysis, and Correlation with Chemical/Microbial Composition: A Pilot Study on Gallstone Geobiology.","authors":"Vipin Yadav, Ragini Kilambi, Rajveer Sharma, Nihar Mohaptra, Shridhar V Sasturkar, Anit Dawar, Archana Rastogi, Manju Saini, Deepti Sharma, Nirupama Trehanpati, Pratibha R Kale, Viniyendra Pamecha, Gayatri Ramakrishna","doi":"10.1016/j.gassur.2024.101937","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>India has a high incidence of gallstones, which can cause chronic inflammation and increase the risk of gallbladder cancer. Understanding the age and composition of gallstones can provide insights into their formation and growth. This study used ¹⁴C dating, FTIR, and metagenome analysis to explore the natural history, deposition rate, and microbial/chemical composition of gallstones.</p><p><strong>Method: </strong>In this pilot study three cholesterol gallstones were chosen with different tissue histopathologies (normal, metaplasia, and dysplasia) and respective layers were analyzed for <sup>14</sup>C bomb pulse dating and FTIR for age and chemical composition respectively. The core of each gallstone was subjected to scanning electron microscopy and further to 16S rRNA sequencing for microbial analysis.</p><p><strong>Result: </strong>The <sup>14</sup>C data indicated that the largest stone with dysplastic epithelium formed over a period of six years, while stones with metaplasia and normal pathology took 13 and 12 years, to develop, respectively. Further, the largest stone was dormant for 6 years before subject experienced acute pain, while other two stones laid dormant for 7 and 18 years. FTIR analysis revealed that all the three gallstones were primarily composed of cholesterol. Additionally, calcium oxalate, calcium carbonate, and calcium bilirubinate were present in stones with underlying dysplasia. The 16S rRNA analysis showed an increased abundance of Corynebacterium sp. in stones associated with metaplasia and dysplasia. Moreover, pathogenic species of Klebsiella and E. coli were abundant in calcium oxalate-rich gallstones with underlying dysplasia.</p><p><strong>Conclusion: </strong>Overall, the pilot study established the feasibility of <sup>14</sup>C bomb pulse in evaluating the timeline of gallstone formation and combining with FTIR/metagenome analysis helps in understanding the natural history of gallstone associated disease.</p>","PeriodicalId":15893,"journal":{"name":"Journal of Gastrointestinal Surgery","volume":" ","pages":"101937"},"PeriodicalIF":2.2000,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Gastrointestinal Surgery","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.gassur.2024.101937","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: India has a high incidence of gallstones, which can cause chronic inflammation and increase the risk of gallbladder cancer. Understanding the age and composition of gallstones can provide insights into their formation and growth. This study used ¹⁴C dating, FTIR, and metagenome analysis to explore the natural history, deposition rate, and microbial/chemical composition of gallstones.
Method: In this pilot study three cholesterol gallstones were chosen with different tissue histopathologies (normal, metaplasia, and dysplasia) and respective layers were analyzed for 14C bomb pulse dating and FTIR for age and chemical composition respectively. The core of each gallstone was subjected to scanning electron microscopy and further to 16S rRNA sequencing for microbial analysis.
Result: The 14C data indicated that the largest stone with dysplastic epithelium formed over a period of six years, while stones with metaplasia and normal pathology took 13 and 12 years, to develop, respectively. Further, the largest stone was dormant for 6 years before subject experienced acute pain, while other two stones laid dormant for 7 and 18 years. FTIR analysis revealed that all the three gallstones were primarily composed of cholesterol. Additionally, calcium oxalate, calcium carbonate, and calcium bilirubinate were present in stones with underlying dysplasia. The 16S rRNA analysis showed an increased abundance of Corynebacterium sp. in stones associated with metaplasia and dysplasia. Moreover, pathogenic species of Klebsiella and E. coli were abundant in calcium oxalate-rich gallstones with underlying dysplasia.
Conclusion: Overall, the pilot study established the feasibility of 14C bomb pulse in evaluating the timeline of gallstone formation and combining with FTIR/metagenome analysis helps in understanding the natural history of gallstone associated disease.
期刊介绍：
The Journal of Gastrointestinal Surgery is a scholarly, peer-reviewed journal that updates the surgeon on the latest developments in gastrointestinal surgery. The journal includes original articles on surgery of the digestive tract; gastrointestinal images; "How I Do It" articles, subject reviews, book reports, editorial columns, the SSAT Presidential Address, articles by a guest orator, symposia, letters, results of conferences and more. This is the official publication of the Society for Surgery of the Alimentary Tract. The journal functions as an outstanding forum for continuing education in surgery and diseases of the gastrointestinal tract.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
