Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2025-12-01 Epub Date: 2024-12-26 DOI:10.1080/13510002.2024.2445398

Amira K Eltokhy, Rehab Ahmed Ahmed El-Shaer, Omnia Safwat El-Deeb, Eman E Farghal, Rowida Raafat Ibrahim, Rasha Elesawy, Marwa Mahmoud Awad, Radwa Ismail, Shaimaa M Motawea, Doaa Shatat, Yasser Mostafa Hafez, Hend Ahmed El Hanafy, Marwa Mohamed Atef

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引用次数: 0

Abstract

Background: The most prevalent endocrine disorder affecting women is PCOS. Programmed death of ovarian cells has yet to be elucidated. Ferroptosis is a kind of iron-dependent necrosis featured by significantly Fe⁺²-dependent lipid peroxidation. The ongoing study aimed to reinforce fertility by combining therapy with AgNPs and (Zileuton) in PCOS rats' model.

Methods: The study included 75 adult female rats divided into 5 groups; control, PCOS, PCOS treated with AgNPs, PCOS treated with Zileuton, and PCOS group treated with AgNPs and Zileuton. The study investigated the anti-ferroptotic, anti-inflammatory, antioxidant, antiapoptotic, histopathological and immunohistochemical examinations of COX-2 and VEGF.

Results: The combination of AgNPs and Zileuton showed significant reduction of inflammatory mediators (IL-6, TNF-α, NFk-B) compared with diseased group (P-value < 0.05), regression of ferroptosis marks (Panx1 and TLR4 expression, Fe⁺² levels) compared with diseased group (P-value < 0.05), depression of apoptotic marker caspase 3 level compared with diseased animals (P-value < 0.05), depression of MDA level, elevation of HO-1, GPx4 activity, and reduction of Cox2 and VEGF as compared with the diseased, AgNPs or zileuton-treated groups (P-value < 0.05).

Conclusion: The study showed that the combination of AgNPs and zileuton guards against, inflammation, apoptosis, and ferroptosis in PCO.

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AgNPs和zileuton通过铁下垂和炎症缓解对PCOS的协同作用。

背景：影响女性最常见的内分泌疾病是多囊卵巢综合征。卵巢细胞的程序性死亡尚未得到阐明。铁下垂是一种铁依赖性坏死，其特征是明显的铁+2依赖性脂质过氧化。正在进行的研究旨在通过AgNPs和（Zileuton）联合治疗PCOS大鼠模型来增强生育能力。方法：选取成年雌性大鼠75只，分为5组；对照组、PCOS组、AgNPs组、Zileuton组、AgNPs组和Zileuton组。研究COX-2和VEGF的抗衰、抗炎、抗氧化、抗凋亡、组织病理学和免疫组化检查。结果：AgNPs与Zileuton联用可显著降低病变组（p值+2个水平）炎性介质（IL-6、TNF-α、NFk-B）水平。结论：AgNPs与Zileuton联用可显著降低PCO中炎性介质（IL-6、TNF-α、NFk-B）水平。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.