A novel microfluidic device for human sperm separation based on rheotaxis.

IF 1.5 4区生物学 Q4 CELL BIOLOGY Zygote Pub Date : 2024-12-27 DOI:10.1017/S0967199424000467

Alireza Heidarnejad, Mohammadreza Sadeghi, Saeid Arasteh, Mohammad Adel Ghiass

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Abstract

This study explores the efficacy of a novel microfluidic device in isolating rheotactic sperm and assesses their advantages compared with other motile sperm. Two microfluidic devices were used in this study: the microfluidic device we designed to separate sperm based on rheotaxis and a simple passive microfluidic device. We compared the results with the density gradient centrifugation technique. Sperm attributes including concentration, morphology, viability and motility were assessed using related procedures. Statistical analyses were conducted using one-way analysis of variance. Results showed differences in sperm concentration, motility, morphology and vitality using different sperm separation techniques. The sperms separated using our microfluidic device demonstrated the highest motilities, normal morphology percentages and higher sperm vitality but significantly lower sperm concentrations. These findings suggest the potential of our microfluidic design in enhancing sperm quality. Our findings are in agreement with previous research, emphasizing the capability of microfluidics in enhancing sperm quality. Specifically, our designed microfluidic device exhibited exceptional efficacy in isolating highly motile sperm, a critical factor for successful fertilization.

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一种基于流变性的人类精子分离微流控装置。

本研究探讨了一种新型微流体装置在分离变性精子中的效果，并评估了其与其他活动精子相比的优势。本研究使用了两种微流控装置：我们设计的基于流变性的精子分离微流控装置和一种简单的无源微流控装置。我们将结果与密度梯度离心技术进行了比较。使用相关程序评估精子属性，包括浓度、形态、活力和活力。统计分析采用单因素方差分析。结果表明，不同的精子分离技术在精子浓度、活力、形态和活力方面存在差异。使用我们的微流体装置分离的精子显示出最高的运动性，正常形态百分比和更高的精子活力，但精子浓度显著降低。这些发现表明我们的微流体设计在提高精子质量方面的潜力。我们的发现与先前的研究一致，强调了微流体在提高精子质量方面的能力。具体来说，我们设计的微流控装置在分离高运动精子方面表现出卓越的功效，这是成功受精的关键因素。

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来源期刊

Zygote 生物-发育生物学

CiteScore

1.70

自引率

5.90%

发文量

117

审稿时长

6-12 weeks

期刊介绍： An international journal dedicated to the rapid publication of original research in early embryology, Zygote covers interdisciplinary studies on gametogenesis through fertilization to gastrulation in animals and humans. The scope has been expanded to include clinical papers, molecular and developmental genetics. The editors will favour work describing fundamental processes in the cellular and molecular mechanisms of animal development, and, in particular, the identification of unifying principles in biology. Nonetheless, new technologies, review articles, debates and letters will become a prominent feature.