3D-printed suction clamps for tensile testing of brain tissue

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2024-12-17 DOI:10.1016/j.jmbbm.2024.106873

J. Zwirner , J.N. Waddell , B. Ondruschka , K.C. Li

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Abstract

The conventional mounting of ultra-soft biological tissues often involves gluing it between two plates or manually tightening grips. Both methods demand delicate handling skills and are time-consuming. This study outlines the design and practical application of 3D-printed suction clamps for uniaxial tension tests on brain samples. Successful testing was defined by the absence of relevant slippage or the sample being drawn into the clamp. A total of 112 deer brain samples underwent testing using a universal testing machine after one freeze-thaw cycle. These samples were obtained from eight different brain regions. During sample preparation, 7 out of all samples failed. Among the 105 tests, 89 (85%) were successful. Of the 16 unsuccessful tests, 15 samples (14%) slipped, while only one sample (1%) was drawn into the clamp to an extent that testing became impossible. Medulla oblongata samples exhibited exceptionally high slippage at 38%, whereas samples from the temporal cortex, external capsule, and putamen had the lowest slippage in only one single case. In conclusion, suction clamps facilitate high-throughput testing through user-friendly and rapid sample mounting. Testing success is contingent on the specific brain site, with sample slippage being the primary reason for testing failures, while sample inspiration into the clamp is negligible.

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用于脑组织拉伸测试的3d打印吸钳。

超软生物组织的传统安装通常需要将其粘在两个板之间或手动拧紧手柄。这两种方法都需要精细的处理技巧，而且耗时。本研究概述了用于脑样本单轴张力测试的3d打印吸钳的设计和实际应用。成功的测试被定义为没有相关的滑移或样品被拉入夹具。112个鹿脑样本经过一个冻融循环后，使用万能试验机进行测试。这些样本来自八个不同的大脑区域。在样品制备过程中，有7个样品不合格。在105次试验中，89次（85%）成功。在16次不成功的测试中，15个样品（14%）滑倒，而只有一个样品（1%）被拉入夹具，以至于无法进行测试。延髓样品的滑移率异常高，达到38%，而颞叶皮层、外包膜和壳核样品的滑移率最低，仅有一例。总之，吸钳通过用户友好和快速的样品安装促进高通量测试。测试成功取决于特定的大脑部位，样品滑动是测试失败的主要原因，而样品注入夹具是可以忽略不计的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.