Jiyeon Hong, Hyeoncheol Francis Son, So-Young Park, Jiyoung Park, Hogyun Seo, Kwang-Hyeon Liu, Kyung-Jin Kim
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引用次数: 0
Abstract
Thiolase is essential in the first step of synthesizing many value-added biochemicals. However, only a few feasible THLs have been reported, and a limited understanding of these enzymes has restricted their broader application in valuable production processes. In this study, biochemical and structural analysis identified the specific properties of THLs belonging to three groups for synthesizing medium- to long-chain fatty acids, polyketide, and dicarboxylic acid, represented by adipic acid. We further demonstrated that hydrolysis activity, considered a rate-limiting step in the biosynthetic process, can be alleviated through flux balance and site-directed mutagenesis. Notably, we discovered two THLs with strong biosynthetic abilities and low hydrolysis: PaTHL4, effective for medium- to long-chain fatty acids and polyketides, and PaTHL7, tailored for adipic acid production. This study provides valuable insights into tailored THLs for specific biochemical production.
期刊介绍：
ACS Catalysis is an esteemed journal that publishes original research in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. It offers broad coverage across diverse areas such as life sciences, organometallics and synthesis, photochemistry and electrochemistry, drug discovery and synthesis, materials science, environmental protection, polymer discovery and synthesis, and energy and fuels.
The scope of the journal is to showcase innovative work in various aspects of catalysis. This includes new reactions and novel synthetic approaches utilizing known catalysts, the discovery or modification of new catalysts, elucidation of catalytic mechanisms through cutting-edge investigations, practical enhancements of existing processes, as well as conceptual advances in the field. Contributions to ACS Catalysis can encompass both experimental and theoretical research focused on catalytic molecules, macromolecules, and materials that exhibit catalytic turnover.
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