Evaluation of a 70 kDa Excreted/Secreted Coproantigen Immunoassay for the Detection of Toxocara canis in Dogs.

IF 0.9 4区医学 Q4 PARASITOLOGY Iranian Journal of Parasitology Pub Date : 2024-10-01 DOI:10.18502/ijpa.v19i4.17162

Ana Cristina González-Morales, Zinnia Judith Molina-Garza, Ricardo Gomez-Flores, Juan José Zárate-Ramos, Lucio Galaviz-Silva

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引用次数: 0

Abstract

Background: We aimed to develop a sandwich ELISA, using polyclonal antibodies against excretory/secretory (E/S) antigens specific to coproantigens present in Toxocara canis-positive dogs.

Methods: Antibodies were produced at Biological Sciences School, Autonomous University of Nuevo León, México in 2023 by immunization of rabbits with antigenic extracts from in vitro cultures of T. canis larvae. Assays were performed on 100 stool samples from pet dogs, measuring sensitivity, specificity, and cross-reactivity against other parasitic infections.

Results: High values of sensitivity (100%), specificity (90.9%), and positive (93.47%) and negative (95.45%) predictive values were obtained, respectively. We obtained an E/S protein with a molecular weight of 70 kDa, which showed high sensitivity and specificity by ELISA, but it presented cross-reactivity against Ancylostoma caninum and Strongyloides stercoralis.

Conclusion: Additional studies are necessary to increase the specificity percentage since this assay demonstrated significant potential as a useful and inexpensive diagnostic tool for immunodiagnostic in dog feces.

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70 kDa排泄/分泌粪原抗原免疫法检测犬弓形虫的评价。

背景：我们的目标是开发一种夹心ELISA，使用针对犬弓形虫阳性犬的粪原抗原特异性排泄/分泌（E/S）抗原的多克隆抗体。方法：采用犬绦虫幼虫体外培养物抗原提取物免疫家兔，于2023年在新自治大学生物科学学院León， m xico。对100个宠物狗粪便样本进行检测，测量敏感性、特异性和对其他寄生虫感染的交叉反应性。结果：获得较高的敏感性（100%）、特异性（90.9%）、阳性预测值（93.47%）和阴性预测值（95.45%）。我们获得了分子量为70 kDa的E/S蛋白，ELISA检测结果显示该蛋白具有较高的敏感性和特异性，但对犬钩虫和粪圆线虫具有交叉反应性。结论：由于该检测显示出作为一种有用且廉价的狗粪便免疫诊断工具的巨大潜力，因此需要进一步的研究来提高特异性百分比。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Parasitology PARASITOLOGY-

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.