Molecular Tracking of Leishmania major in an Archived Rattus norvegicus Spleen Sample in Iran: A Case Report.

IF 0.9 4区 医学 Q4 PARASITOLOGY Iranian Journal of Parasitology Pub Date : 2024-10-01 DOI:10.18502/ijpa.v19i4.17172
Mahya Allahmoradi, Faezeh Najafi, Mona Koosha, Elham Kazemirad, Alireza Latifi, Saied Reza Naddaf, Hamed Mirjalali, Mehdi Mohebali, Homa Hajjaran, Domenico Otranto, Gholamreza Mowlavi
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Abstract

Rodents are the primary reservoir hosts for zoonotic cutaneous leishmaniasis (ZCL) caused by Leishmania major. Knowing reservoir hosts is crucial for leishmaniasis surveillance and control programs in endemic areas. In this study, we examined an archived spleen of Rattus norvegicus obtained during a pest control program in 2000 in Tehran, the capital of Iran. The sample was analyzed using polymerase chain reaction (PCR) and sequencing to determine the presence of Trypanosomatidae based on the internal transcribed spacer (ITS) 1 gene. Amplification and sequencing of the discriminative region of the ITS1 gene followed by BLAST analysis showed the highest similarity with L. major isolates. Also, the phylogenetic analysis revealed that our sample was grouped with L. major isolates retrieved from the GenBank database. This finding might support the claim that R. norvegicus acts as a potential reservoir host for L. major. Further studies, including a survey on more rodent samples as well as studying sandflies in the area, might uncover the possible presence of such pathobiological conditions in ZCL transmission in urban and suburban settings.

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伊朗褐家鼠脾标本中主要利什曼原虫的分子追踪:1例报告。
啮齿动物是主要利什曼原虫引起的人畜共患皮肤利什曼病的主要宿主。了解宿主对利什曼病流行地区的监测和控制规划至关重要。在这项研究中,我们检查了2000年在伊朗首都德黑兰虫害防治项目中获得的褐家鼠脾脏。采用聚合酶链式反应(PCR)和测序技术,根据ITS - 1基因分析样品是否存在锥虫病。对ITS1基因的鉴别区进行扩增和测序,并进行BLAST分析,结果表明其与L. major菌株的相似性最高。此外,系统发育分析显示,我们的样本与从GenBank数据库检索到的L. major分离株属于同一组。这一发现可能支持褐家鼠作为大褐家鼠潜在宿主的说法。进一步的研究,包括对更多啮齿动物样本的调查以及对该地区白蛉的研究,可能会发现在城市和郊区环境中ZCL传播中可能存在这种病理生物学条件。
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来源期刊
CiteScore
1.80
自引率
0.00%
发文量
55
审稿时长
6-12 weeks
期刊介绍: Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members. The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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