Detection of elevated succinate in brain during circulatory arrest in a piglet model: A 3T ¹H MR spectroscopy study.

IF 3 3区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Magnetic Resonance in Medicine Pub Date : 2024-12-31 DOI:10.1002/mrm.30414

Ralph E Hurd, Meng Gu, Kenichi Okamura, Masafumi Shibata, Yoshikazu Ono, Moussa Haidar, R Kirk Riemer, Frank L Hanley, Daniel M Spielman

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Abstract

Purpose: To measure and validate elevated succinate in brain during circulatory arrest in a piglet model of cardiopulmonary bypass.

Methods: Using data from an archive of 3T ¹H MR spectra acquired in previous in-magnet studies, dynamic plots of succinate, spectral simulations and difference spectra were generated for analysis and validation.

Results: Elevation of succinate during circulatory arrest was observed and validated. Fitting bias was evaluated as a function of the line-widths and signal-to-noise ratios of the archived data. Succinate increases were independent of bypass temperature. Succinate elevation was also not observed with antegrade cerebral perfusion.

Conclusion: Although spectrally overlapped and at sub-millimolar levels, elevated brain succinate can be reliably measured by dynamic MR spectroscopy at 3T. Noise dependent bias of the stronger overlapping signals did not impact the succinate measurement. Elevated succinate during circulatory arrest and its recovery after reperfusion was observed. This finding is consistent with earlier reports that correlate elevated succinate with ischemic-reperfusion injury.

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在仔猪模型中检测循环停止时脑中升高的琥珀酸：3T 1H MR 光谱研究。

目的：测定和验证体外循环仔猪模型循环停搏时脑内琥珀酸盐的升高。方法：利用以往在磁体内研究中获得的3T 1H磁共振光谱档案数据，生成琥珀酸盐的动态图、光谱模拟和差异光谱，进行分析和验证。结果：观察并验证了循环骤停时琥珀酸盐升高。将拟合偏差作为存档数据的线宽和信噪比的函数进行评估。琥珀酸盐的增加与旁路温度无关。顺行脑灌注组也未观察到琥珀酸盐升高。结论：虽然光谱重叠且处于亚毫摩尔水平，但动态磁共振光谱在3T时可以可靠地测量脑琥珀酸升高。较强重叠信号的噪声依赖偏置不影响琥珀酸盐测量。循环骤停时琥珀酸盐升高，再灌注后恢复。这一发现与早期报道的琥珀酸盐升高与缺血-再灌注损伤相关一致。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Magnetic Resonance in Medicine 医学-核医学

CiteScore

6.70

自引率

24.20%

发文量

376

审稿时长

2-4 weeks

期刊介绍： Magnetic Resonance in Medicine (Magn Reson Med) is an international journal devoted to the publication of original investigations concerned with all aspects of the development and use of nuclear magnetic resonance and electron paramagnetic resonance techniques for medical applications. Reports of original investigations in the areas of mathematics, computing, engineering, physics, biophysics, chemistry, biochemistry, and physiology directly relevant to magnetic resonance will be accepted, as well as methodology-oriented clinical studies.