Toxicological investigation of bisphenol A and its derivates on human breast epithelial (MCF-10A) cells.

IF 2.6 3区医学 Q3 TOXICOLOGY Toxicology in Vitro Pub Date : 2024-12-28 DOI:10.1016/j.tiv.2024.106004

Buket Bakan, Burak Kaptaner, Merve Tokmak, Handan Aykut, Ali Sefa Mendil, Mustafa Özkaraca

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Abstract

Bisphenols can enter the body, where they have potential adverse effects on human health, via different routes such as inhalation, dermally or orally. They are known as endocrine disrupting chemicals that activate signaling pathways by mimicking the estrogen actions. In this study, we aimed to investigate effects of bisphenol A (BPA), and its analogues bisphenol F (BPF) and bisphenol S (BPS) on MCF-10A cells and their impact mechanisms on autophagy, apoptosis and reduced glutathion levels. In comparison of the cytotoxic effects, while BPF and BPS showed dose-dependent high toxicity on MCF-10A cells, BPA exerted cytotoxic effects only at the highest doses. Caspase 3 and LC3B are strongly and positively correlated with BPF exposures while significant changes were not detected in the BPA and BPS applied groups. It was clearly observed that BPF and BPS displayed more toxic effects than BPA on human breast cells that are important targets for the bisphenols. These findings provide data for understanding the mechanisms for BPA, BPF and BPS-induced toxicity on human breast cells.

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双酚 A 及其衍生物对人类乳腺上皮细胞 (MCF-10A) 的毒理学研究。

双酚可通过吸入、皮肤或口服等不同途径进入人体，对人体健康产生潜在的不利影响。众所周知，双酚 A 是一种干扰内分泌的化学物质，它通过模仿雌激素的作用来激活信号通路。在这项研究中，我们旨在调查双酚 A（BPA）及其类似物双酚 F（BPF）和双酚 S（BPS）对 MCF-10A 细胞的影响，以及它们对自噬、细胞凋亡和谷胱甘肽水平降低的影响机制。在细胞毒性效应比较中，双酚 F 和双酚 S 对 MCF-10A 细胞的毒性呈剂量依赖性，而双酚 A 仅在最高剂量时才产生细胞毒性效应。Caspase 3 和 LC3B 与暴露于 BPF 强烈正相关，而在暴露于 BPA 和 BPS 的组别中未检测到显著变化。可以清楚地观察到，与双酚 A 相比，BPF 和 BPS 对人类乳腺细胞的毒性作用更大，而乳腺细胞是双酚 A 的重要靶标。这些发现为了解双酚 A、双酚 F 和双酚 S 诱导人类乳腺细胞毒性的机制提供了数据。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.