StarTrack: Mapping Cellular Fates with Inheritable Color Codes.

Q4 Biochemistry, Genetics and Molecular Biology Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4310-5_16

M Figueres-Oñate, Jorge García-Marqués, A C Ojalvo-Sanz, Laura López-Mascaraque

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Abstract

StarTrack is a powerful multicolor genetic tool designed to unravel cellular lineages arising from neural progenitor cells (NPCs). This innovative technique, based on retrospective clonal analysis and built upon the PiggyBac system, creates a unique and inheritable "color code" within NPCs. Through the stochastic integration of 12 distinct plasmids encoding six fluorescent proteins, StarTrack enables precise and comprehensive tracking of cellular fates and progenitor potentials. The versatility of this tool is further enhanced by the potential of combining multiple promoters. Whether through the use of fluorescent integrable constructs or driving the expression of the PiggyBac transposase, StarTrack broadens the horizons for lineage tracing from progenitors of multiple origins.StarTrack revolutionized our understanding of cellular origins and lineages, offering an invaluable resource for researchers in the field of neural development and lineage tracing. This protocol provides a comprehensive overview of the technique's capabilities and applications, shedding light on its significance within the scientific community.

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星轨用可遗传的颜色编码绘制细胞命运图谱

StarTrack是一种功能强大的多色遗传工具，旨在揭示神经祖细胞（npc）产生的细胞谱系。这种基于回顾性克隆分析并建立在PiggyBac系统上的创新技术，在npc中创建了独特且可继承的“颜色代码”。通过随机整合12个不同的编码6种荧光蛋白的质粒，StarTrack能够精确和全面地跟踪细胞命运和祖细胞电位。结合多个启动子的潜力进一步增强了该工具的多功能性。无论是通过使用荧光可整合结构还是驱动PiggyBac转座酶的表达，StarTrack都拓宽了从多个起源的祖细胞进行谱系追踪的视野。StarTrack彻底改变了我们对细胞起源和谱系的理解，为神经发育和谱系追踪领域的研究人员提供了宝贵的资源。该协议提供了该技术的能力和应用的全面概述，阐明了其在科学界的重要性。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.