Mixed-species Pseudomonas biofilms: a novel and sustainable strategy for malachite green dye decolorization and detoxification.

IF 2.4 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Folia microbiologica Pub Date : 2025-01-02 DOI:10.1007/s12223-024-01238-0

Nabanita Ghosh, Arup Ratan Biswas, Arindam Chakraborty, Arnab Ganguli

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Abstract

This study investigated the application of mixed biofilms formed by two Pseudomonas strains (NAA22 and NAA23) for bio-decolorization of malachite green (MG) dye. The isolated strains displayed biofilm formation and MG decolorization capabilities. Mixed biofilms exhibited significantly greater biofilm formation and MG decolorization (94.3%) compared to individual strains, suggesting synergistic interactions. This decolorization efficiency surpassed previously reported values for single strain decolorization. The mixed biofilms tolerated a broad range of temperatures (20-40 °C) and pH (5-9), with optimal decolorization at neutral or slightly acidic conditions (pH7.0). Enzyme analysis revealed laccase, NADH-DCIP reductase, and azoreductase as key contributors to MG decolorization, with significantly higher activity in mixed biofilms. Importantly, the bio-decolorization process transformed MG into non-phytotoxic compounds, demonstrated by seed germination and growth assays. These findings propose a promising and environmentally safe approach for MG bioremediation using mixed Pseudomonas biofilms.

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混合种假单胞菌生物膜：孔雀石绿染料脱色和解毒的一种新颖和可持续的策略。

研究了由NAA22和NAA23假单胞菌组成的混合生物膜对孔雀石绿染料的生物脱色效果。分离菌株表现出生物膜形成和MG脱色能力。与单个菌株相比，混合生物膜的生物膜形成和MG脱色率显著提高（94.3%），表明两者之间存在协同作用。这种脱色效率超过了以前报道的单菌株脱色值。混合生物膜可耐受广泛的温度（20-40℃）和pH(5-9)，在中性或微酸性条件下（pH7.0）脱色效果最佳。酶分析表明，漆酶、NADH-DCIP还原酶和偶氮还原酶是MG脱色的关键酶，在混合生物膜中活性显著提高。重要的是，通过种子萌发和生长试验，生物脱色过程将MG转化为非植物毒性化合物。这些发现为使用混合假单胞菌生物膜进行MG生物修复提供了一种有前景且环境安全的方法。

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来源期刊

Folia microbiologica 工程技术-生物工程与应用微生物

CiteScore

5.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.