Selenocysteine-Activatable Near-Infrared Fluorescent Probe for Screening of Anti-inflammatory Components in Herbs

IF 6.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2025-01-04 DOI:10.1021/acs.analchem.4c02157
Hao Liu, Wangning Zhang, Sisi Wang, Qilin Zhou, Na Xu, Wenze Zhang, Haojiang Ren, Min Yang, Hua Lu, Xianchuang Zheng, Jiangwei Tian
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Abstract

Inflammation, a central process in numerous diseases, plays a crucial role in hepatic disorders, arthritis, cardiac conditions, and neurodegenerative ailments. Given the lack of effective anti-inflammatory drugs, it is imperative to assess inflammation severity and explore novel therapeutics. Selenocysteine (Sec), a key mediator of selenium’s biological function, is closely involved in anti-inflammatory responses. We have synthesized a novel near-infrared fluorescent probe, Sec-BDP, which can image Sec dynamics in vivo with high selectivity and sensitivity. Sec-BDP detects Sec at concentrations as low as 0.085 μM. Utilizing this probe, we visualized Sec levels in cell, zebrafish, and mouse inflammation models, enabling a clear assessment of inflammation severity. To screen for drug candidates, Sec-BDP was integrated with ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry to identify potent anti-inflammatory compounds in Astragalus membranaceus, such as 5-O-methylvisammioside. Imaging of Sec with Sec-BDP provides insights into Sec-related diseases and aids in discovering new treatments. This probe advances selenium biology and promises more targeted therapeutic strategies.

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硒半胱氨酸活化近红外荧光探针筛选中草药抗炎成分
炎症是许多疾病的中心过程,在肝脏疾病、关节炎、心脏病和神经退行性疾病中起着至关重要的作用。鉴于缺乏有效的抗炎药物,评估炎症严重程度和探索新的治疗方法势在必行。硒半胱氨酸(Sec)是硒的生物学功能的关键介质,密切参与抗炎反应。我们合成了一种新型的近红外荧光探针Sec- bdp,该探针具有高选择性和高灵敏度,可以对体内Sec动态进行成像。Sec- bdp检测Sec浓度低至0.085 μM。利用这种探针,我们可视化了细胞、斑马鱼和小鼠炎症模型中的Sec水平,从而能够清楚地评估炎症的严重程度。为了筛选候选药物,Sec-BDP与超高高效液相色谱四极杆飞行时间质谱相结合,以鉴定黄芪中有效的抗炎化合物,如5- o -甲基维沙米苷。用Sec- bdp成像Sec提供了对Sec相关疾病的深入了解,并有助于发现新的治疗方法。该探针推进了硒生物学,并承诺更有针对性的治疗策略。
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来源期刊
Analytical Chemistry
Analytical Chemistry 化学-分析化学
CiteScore
12.10
自引率
12.20%
发文量
1949
审稿时长
1.4 months
期刊介绍: Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
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