Visualization of the protective role of estrogen against female liver fibrosis via an ER viscosity NIR fluorescent probe

IF 10.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Science China Chemistry Pub Date : 2024-10-23 DOI:10.1007/s11426-024-2061-7

Zheng Liu, Changyu Zhang, Zipeng Li, Wanying Ma, Jia Liu, Xiang Xia, Ning Xu, Wen Sun, Jianjun Du, Jiangli Fan, Xiaojun Peng

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Abstract

Endoplasmic reticulum (ER) viscosity has emerged as a new potential biomarker for hepatic fibrosis (HF). Herein, we report the first ER-targeting viscosity-responsive near-infrared (NIR) fluorescent probe HBT-PP for investigations of HF pathogenesis. The twisted intramolecular charge transfer (TICT) and the excited state intramolecular proton transfer (ESIPT) are combined to endow HBT-PP with the selective response towards viscosity (∼62-fold) and a super large stokes shift (∼320 nm). HBT-PP mainly targets organelle ER and is able to image the drug- or alcohol-induced viscosity elevation in living cells. Significantly, HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression. Of note, after 10 min of stain with HBT-PP, HF lesions showed a stage-based response, indicating the potential of this probe in fast and early diagnosis of HF. These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.

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雌激素对女性肝纤维化的保护作用通过内质网黏度近红外荧光探针可视化

内质网（ER）黏度已成为肝纤维化（HF）的一种新的潜在生物标志物。在此，我们报道了首个er靶向黏度响应型近红外荧光探针hpt - pp，用于研究HF发病机制。旋扭分子内电荷转移（TICT）和激发态分子内质子转移（ESIPT）相结合，使HBT-PP具有对粘度（~ 62倍）和超大斯托克斯位移（~ 320 nm）的选择性响应。hpt - pp主要靶向细胞器内质网，能够成像药物或酒精诱导的活细胞粘度升高。值得注意的是，hpt - pp成功应用于观察HF的性别差异以及雌激素在抑制HF进展中的保护作用。值得注意的是，在hpt - pp染色10分钟后，HF病变显示出基于阶段的反应，这表明该探针在快速和早期诊断HF方面具有潜力。这些结果清楚地表明，HBT-PP作为探索内质网黏度相关生理和病理过程的新工具具有广阔的应用前景。

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来源期刊

Science China Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

7.30%

发文量

3787

审稿时长

2.2 months

期刊介绍： Science China Chemistry, co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China and published by Science China Press, publishes high-quality original research in both basic and applied chemistry. Indexed by Science Citation Index, it is a premier academic journal in the field. Categories of articles include: Highlights. Brief summaries and scholarly comments on recent research achievements in any field of chemistry. Perspectives. Concise reports on thelatest chemistry trends of interest to scientists worldwide, including discussions of research breakthroughs and interpretations of important science and funding policies. Reviews. In-depth summaries of representative results and achievements of the past 5–10 years in selected topics based on or closely related to the research expertise of the authors, providing a thorough assessment of the significance, current status, and future research directions of the field.