Detection of Rocahepevirus ratti in Bivalve Mollusks from São Luís Island, Maranhão, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

IF 2.7 2区农林科学 Q2 ENVIRONMENTAL SCIENCES Food and Environmental Virology Pub Date : 2025-01-04 DOI:10.1007/s12560-024-09624-0

Andreza Soriano Figueiredo, Isabella Rodrigues Negreiros, Aldaleia do Nascimento e Silva, Caroline Roberta Soares Salgado, Natália Lourenço dos Santos, Marcelo Alves Pinto, Alcina Vieira de Carvalho Neta, José Paulo Gagliardi Leite, Carina Pacheco Cantelli

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Abstract

The attempt to investigate hepatitis E virus (HEV) contamination in naturally growing mangrove bivalve mollusks captured for local sale in a touristic area of Maranhão state in Brazil revealed the detection of rat hepatitis E virus (ratHEV). Using international standard protocols for processing and nucleic acid extraction, we analyzed 89 bivalve samples (Mytella falcata and Crassostrea rhizophorae) with two broadly reactive assays: heminested pan-Hepeviridae (ORF-1) and probe-based HEV-1 to HEV-4 (ORF-2/ORF-3). Heminested reactions presented 2 (2.2%) amplifications of the expected size. Nucleotide identities ranged from 86.6 to 89.0% with ratHEV isolates from wild rats, pigs, one human case and sewage reported in different countries. Regarding the phylogenetic tree, the sequences grouped with genotype HEV-C1. This first report of ratHEV detection in bivalve mollusks may be a starting point for further research on virus variability, distribution, host range and especially the possible role of contaminated shellfish as a vehicle for ratHEV transmission.

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巴西maranh岛s o Luís双壳类软体动物中鼠形罗卡疱疹病毒的检测：一种新出现的人畜共患病原体的潜在传播途径？

在巴西maranh州一个旅游区捕获的用于当地销售的自然生长的红树林双壳类软体动物中，调查戊型肝炎病毒（HEV）污染的尝试发现了大鼠戊型肝炎病毒（ratHEV）。采用国际标准的处理和核酸提取方法，对89份双壳类样品（falcata Mytella和Crassostrea rhizophorae）进行了两种广泛反应性分析：半隐式泛hepeviridae （ORF-1）和基于探针的HEV-1至HEV-4 （ORF-2/ORF-3）。半嵌套反应呈现预期大小的2倍（2.2%）放大。从不同国家报告的野生大鼠、猪、1例人类病例和污水中分离出的病毒的核苷酸同源性为86.6 ~ 89.0%。在系统发育树上，这些序列归为HEV-C1基因型。这是在双壳类软体动物中检测到甲状病毒的第一份报告，可能是进一步研究甲状病毒变异、分布、宿主范围，特别是受污染的贝类作为甲状病毒传播媒介的可能作用的起点。

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来源期刊

Food and Environmental Virology ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.50

自引率

2.90%

发文量

审稿时长

1 months

期刊介绍： Food and Environmental Virology publishes original articles, notes and review articles on any aspect relating to the transmission of pathogenic viruses via the environment (water, air, soil etc.) and foods. This includes epidemiological studies, identification of novel or emerging pathogens, methods of analysis or characterisation, studies on survival and elimination, and development of procedural controls for industrial processes, e.g. HACCP plans. The journal will cover all aspects of this important area, and encompass studies on any human, animal, and plant pathogenic virus which is capable of transmission via the environment or food.