High-alcohol-producing Klebsiella pneumoniae aggravates lung injury by affecting neutrophils and the airway epithelium.

IF 11.7 1区医学 Q1 CELL BIOLOGY Cell Reports Medicine Pub Date : 2025-01-21 Epub Date: 2025-01-02 DOI:10.1016/j.xcrm.2024.101886

Jinghua Cui, Ziying Xu, Zihui Yu, Qun Zhang, Shiyu Liu, Bing Du, Lin Gan, Chao Yan, Guanhua Xue, Junxia Feng, Zheng Fan, Tongtong Fu, Yanling Feng, Hanqing Zhao, Zanbo Ding, Xiaoran Li, Rui Zhang, Xiaohu Cui, Ziyan Tian, Kewu Huang, Wenjun Wang, Yu Bai, Haijian Zhou, Ying Sun, Xiaopeng Yang, Meng Wan, Yuehua Ke, Jing Yuan

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Abstract

We have previously reported that high-alcohol-producing Klebsiella pneumoniae (HiAlc Kpn) in the gut can cause endo-alcoholic fatty liver disease. Here, we discover that 91.2% of Kpn isolates from pulmonary disease samples also produce excess ethanol, which may be associated with respiratory disease severity. To further explore the potential mechanism, a murine model is established with high-dose bacteria. Kpn stimulates granular neutrophils (G0), subsequently transforming them into phagocytic neutrophils (G1). HiAlc Kpn also causes dysfunction of pyrimidine metabolism, leading to neutrophil apoptosis. These changes inhibit phagocytosis of neutrophils and possibly suppress inflammasome-dependent innate immunity. In a persistent infective murine model, HiAlc Kpn induces lung fibrosis and production of reactive oxygen species (ROS), possibly affecting epithelial cell apoptosis and lung function. The results suggest that the subtype of neutrophil is a potential biomarker for the severity of lung injury caused by HiAlc Kpn.

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高酒精产生肺炎克雷伯菌通过影响中性粒细胞和气道上皮加重肺损伤。

我们以前曾报道过，肠道内高酒精生成肺炎克雷伯菌（HiAlc Kpn）可引起内酒精性脂肪肝。在这里，我们发现从肺部疾病样本中分离的91.2%的Kpn也产生过量的乙醇，这可能与呼吸道疾病的严重程度有关。为了进一步探索其潜在的机制，我们用大剂量细菌建立了小鼠模型。Kpn刺激颗粒中性粒细胞（G0），随后将其转化为吞噬性中性粒细胞（G1）。HiAlc Kpn还引起嘧啶代谢功能障碍，导致中性粒细胞凋亡。这些变化抑制了嗜中性粒细胞的吞噬，并可能抑制炎性小体依赖性先天免疫。在持续感染小鼠模型中，HiAlc Kpn诱导肺纤维化和活性氧（ROS）的产生，可能影响上皮细胞凋亡和肺功能。结果提示，嗜中性粒细胞亚型是HiAlc Kpn所致肺损伤严重程度的潜在生物标志物。

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来源期刊

Cell Reports Medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

15.00

自引率

1.40%

发文量

231

审稿时长

40 days

期刊介绍： Cell Reports Medicine is an esteemed open-access journal by Cell Press that publishes groundbreaking research in translational and clinical biomedical sciences, influencing human health and medicine. Our journal ensures wide visibility and accessibility, reaching scientists and clinicians across various medical disciplines. We publish original research that spans from intriguing human biology concepts to all aspects of clinical work. We encourage submissions that introduce innovative ideas, forging new paths in clinical research and practice. We also welcome studies that provide vital information, enhancing our understanding of current standards of care in diagnosis, treatment, and prognosis. This encompasses translational studies, clinical trials (including long-term follow-ups), genomics, biomarker discovery, and technological advancements that contribute to diagnostics, treatment, and healthcare. Additionally, studies based on vertebrate model organisms are within the scope of the journal, as long as they directly relate to human health and disease.