Effect of zoledronic acid on biological characteristics of cervical cancer cells.

Abstract

Cervical cancer (CC) is a malignant tumor in females characterized by high incidence and mortality rates, often resulting in a poor prognosis for patients. Zoledronic acid (ZA), a third-generation bisphosphonate, exhibits anti-tumor properties across various types of tumors. To further understand the effect of ZA in the treatment of CC, this article included two kinds of human CC cells (CCCs) as the research object, examining the impact of varying levels of ZA on the cells' biological properties. Hela and Siha were cultivated and exposed to ZA at 0, 50, 100, and 200 μM, and the changes of cell proliferation, clone formation, migration, and invasion characteristics were detected. Cell RNA was extracted to detect epithelial-mesenchymal transition (EMT) and the relative expression (RE) of AKT/GSK3β/β-catenin (β-cat) pathway related proteins. The results show that as against 0 μM, the proliferation rate, clone formation cell number, migration distance, and invasive cell number of Hela and Siha were markedly reduced, while E-cadherin (E-cad) was markedly enhanced. N-cadherin (N-cad), vimentin (Vim), p-AKT, p-GSK3β, and β-cat were markedly decreased at 50, 100, and 200 μM ZA; With the increase of ZA concentration, the biological characteristics and protein expression levels of Hela and Siha changed more markedly, showing concentration dependent characteristics (P < 0.05). It was concluded that ZA can influence the malignant biological activities of CCCs.