A New Method of Intraoperative Modeling of Liver Cirrhosis in an Experiment.

Abstract

A method of intraoperative modeling of liver cirrhosis in an experiment has been developed. Mature male Wistar rats underwent typical liver resection (~70% of the initial volume). After resection, intrahepatic injection of 1 ml ademetionine was performed (controls received 0.9% NaCl in the same volume). On days 1, 5, 7, and 14 after surgery, the blood and tissue samples were taken, then the animals were euthanized. Laboratory tests revealed significant deviations in the indicators of cytolysis, cholestasis, and liver cell insufficiency syndromes in the experimental group. Morphological study showed a decrease in the number of mitoses, the development of fibrotic, cirrhotic, and inflammatory changes in the liver. After intrahepatic administration of ademetionine, significant liver dysfunction was observed that did not recover by day 14 after resection, pronounced shifts in laboratory parameters, as well as the development of fibrous, cirrhotic, and inflammatory liver changes.