Stratification of Experimental LPS-Induced Systemic Inflammatory Response by Expression Level of Hif1a and NFkb Genes.

Abstract

It was previously found that the severity of LPS-induced systemic inflammatory response (SIRS) in rats is determined by resistance to hypoxia and the level of Hif1a expression. Individual differences in the level of Hif1a and NFkb expression in the liver were studied in relation to the severity of inflammatory and immune reactions in LPS-induced SIRS in rats without previous placement in a ventilated decompression chamber. During the early periods after SIRS modeling, rats with high expression of the Hif1a and NFkb genes associated with increased expression of pro- and anti-inflammatory cytokines are identified. These animals have a high blood level of corticosterone, low number of neutrophils in the interalveolar septa, and a predominance of T cells over B cells in the peripheral blood. The obtained data can be used to develop new approaches to the individual prediction of the severity of sepsis and SIRS in intensive care units, which will increase the effectiveness of therapy and reduce mortality rate.