Surface interactions of gelatin-sourced carbon quantum dots with a model globular protein: insights into carbon-based nanomaterials and biological systems.

IF 4.6 3区 材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Nanoscale Advances Pub Date : 2024-12-19 DOI:10.1039/d4na00842a
Shima Masoudi Asil, Mahesh Narayan
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Abstract

Carbon nanomaterials (CNMs), such as carbon nanotubes (CNTs), graphene quantum dots (GQDs), and carbon quantum dots (CQDs), are prevalent in biological systems and have been widely utilized in applications like environmental sensing and biomedical fields. While their presence in human matrices is projected to increase, the interfacial interactions between carbon-based nanoscopic platforms and biomolecular systems continue to remain underexplored. In this study, we investigated the effect of gelatin-sourced CQDs on the globular milk protein beta-lactoglobulin (BLG). Exposure to the CQDs resulted in the disruption of BLG's tertiary and secondary structural elements (transformation of isolated helices to coiled-coils and increased beta-sheet content), with IR amide backbone signatures further confirming CQD-induced alterations in protein structures. Importantly, the structural perturbations induced by CQDs compromised BLG : retinol interactions, potentially affecting its physiological ligand transport function. By contrast, cytotoxicity analyses revealed a high viability of neuroblastoma cells exposed to this CNM, suggesting biomolecule-specific effects. Collectively, the data reveal aberrant molecular and functional consequences associated with the interactions of a globular protein with an otherwise biocompatible CQD. In conclusion, this work represents the initial steps toward a comprehensive understanding at the atomic and molecular levels of the outcomes linked to the utilization of carbon-based nanomaterials and their potential adverse systemic consequences.

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明胶源碳量子点与模型球状蛋白的表面相互作用:对碳基纳米材料和生物系统的见解。
碳纳米材料(CNMs),如碳纳米管(CNTs)、石墨烯量子点(GQDs)和碳量子点(CQDs)在生物系统中广泛存在,并在环境传感和生物医学等领域得到广泛应用。虽然它们在人体基质中的存在预计会增加,但碳基纳米平台与生物分子系统之间的界面相互作用仍未得到充分探索。在这项研究中,我们研究了明胶来源的CQDs对球状乳蛋白-乳球蛋白(BLG)的影响。暴露于cqd导致BLG的三级和二级结构元件的破坏(孤立螺旋转化为线圈和β -sheet含量增加),IR酰胺主链特征进一步证实了cqd诱导的蛋白质结构改变。重要的是,CQDs引起的结构扰动损害了BLG:视黄醇的相互作用,潜在地影响了其生理配体运输功能。相比之下,细胞毒性分析显示,神经母细胞瘤细胞暴露于这种CNM具有很高的活力,表明其具有生物分子特异性作用。总的来说,这些数据揭示了与球状蛋白与生物相容性CQD相互作用相关的异常分子和功能后果。总之,这项工作代表了在原子和分子水平上全面理解与碳基纳米材料利用相关的结果及其潜在的不利系统后果的初步步骤。
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来源期刊
Nanoscale Advances
Nanoscale Advances Multiple-
CiteScore
8.00
自引率
2.10%
发文量
461
审稿时长
9 weeks
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