Anticancer potentialsof chaga and notoginseng against dog bladder cancer organoids.

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES Journal of Veterinary Medical Science Pub Date : 2025-01-03 DOI:10.1292/jvms.24-0258
Yuta Shinohara, Mohamed Elbadawy, Yishan Liu, Megumi Yamanaka, Haru Yamamoto, Yomogi Sato, Amira Augomaa, Yusuke Ishihara, Tatsuya Usui, Kazuaki Sasaki
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Abstract

Muscle-invasive bladder cancer (MIBC) is a common form of BC in dogs. Adjuvant chemotherapy administration is commonly applied in MIBC cases, but patients sometimes experience treatment failure and recurrence. Therefore, supplements with anticancer properties, such as traditional Chinese medicines (TCMs), are required, and they have been widely used in Japanese human medicine and may be useful in veterinary medicine. Furthermore, organoid cultures can mimic the characteristics of their original tissues, such as self-renewal and organization. We previously established a novel experimental model for MIBC using a dog BC organoid (DBCO) culture. Herein, we examined the antiproliferative effects and mechanisms of 39 substances, consisting of TCMs, TCM supplements, and crude drug extracts, on DBCOs. Among the TCMs, D3 (also known as Shibe-ria), which is a mixture of chaga (Inonotus obliquus) and notoginseng (Panax notoginseng), significantly diminished the cell viability of DBCOs. The expression of BC stem cell markers, CD44 and SOX2, was reduced considerably in the D3-treated DBCOs. Among the components of D3, chaga exerted an antiproliferative effect on DBCO, whereas notoginseng did not. The administration of D3 also significantly reduced the volume of DBCO xenografted tumors in mice in vivo. Overall, D3 may have benefits as a natural anticancer supplement in veterinary medicine.

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来源期刊
Journal of Veterinary Medical Science
Journal of Veterinary Medical Science 农林科学-兽医学
CiteScore
2.30
自引率
8.30%
发文量
230
审稿时长
9-18 weeks
期刊介绍: JVMS is a peer-reviewed journal and publishes a variety of papers on veterinary science from basic research to applied science and clinical research. JVMS is published monthly and consists of twelve issues per year. Papers are from the areas of anatomy, physiology, pharmacology, toxicology, pathology, immunology, microbiology, virology, parasitology, internal medicine, surgery, clinical pathology, theriogenology, avian disease, public health, ethology, and laboratory animal science. Although JVMS has played a role in publishing the scientific achievements of Japanese researchers and clinicians for many years, it now also accepts papers submitted from all over the world.
期刊最新文献
Acanthomatous ameloblastoma with mucinous glandular differentiation in a dog. Anticancer potentialsof chaga and notoginseng against dog bladder cancer organoids. Colostomies for two lizards with cloacal prolapse. Current status of the distribution and antimicrobial resistance of Staphylococcus felis isolates from feline patients in Japan. A clinical case of CACNA1S-related muscle weakness in a Holstein calf with congenital astasia diagnosed by a genotyping test of stored blood.
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