Liwei Liu, Yanzhu Zhang, Jouni Jokela, Dacheng Wang, Matti Wahlsten, C. Benjamin Naman, Yanrong Li, Shan He
{"title":"Neptunizhulides, Cryptic trans-AT Polyketide Synthase-Derived Metabolites from Paraneptunicella aestuarii NBU2194","authors":"Liwei Liu, Yanzhu Zhang, Jouni Jokela, Dacheng Wang, Matti Wahlsten, C. Benjamin Naman, Yanrong Li, Shan He","doi":"10.1021/acs.orglett.4c03801","DOIUrl":null,"url":null,"abstract":"Genome mining of <i>Paraneptunicella aestuarii</i> NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A–F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by <i>J</i>-based configuration analysis, ROESY NMR, Mosher’s ester derivatization, and bioinformatic predictions. Bioinformatic analysis and a <sup>15</sup>N-serine labeling experiment resulted in the identification of a putative <i>trans</i>-acyltransferase polyketide synthase (<i>trans</i>-AT PKS) biosynthetic gene cluster, and a biosynthetic pathway is proposed.","PeriodicalId":54,"journal":{"name":"Organic Letters","volume":"5 1","pages":""},"PeriodicalIF":4.9000,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Organic Letters","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.orglett.4c03801","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ORGANIC","Score":null,"Total":0}
引用次数: 0
Abstract
Genome mining of Paraneptunicella aestuarii NBU2194 resulted in the identification of a family of 17-membered macrolides, neptunizhulides A–F. Their structures were elucidated by comprehensive spectroscopic data analysis. Stereochemical assignments of the neptunizhulides were determined by J-based configuration analysis, ROESY NMR, Mosher’s ester derivatization, and bioinformatic predictions. Bioinformatic analysis and a 15N-serine labeling experiment resulted in the identification of a putative trans-acyltransferase polyketide synthase (trans-AT PKS) biosynthetic gene cluster, and a biosynthetic pathway is proposed.
期刊介绍：
Organic Letters invites original reports of fundamental research in all branches of the theory and practice of organic, physical organic, organometallic,medicinal, and bioorganic chemistry. Organic Letters provides rapid disclosure of the key elements of significant studies that are of interest to a large portion of the organic community. In selecting manuscripts for publication, the Editors place emphasis on the originality, quality and wide interest of the work. Authors should provide enough background information to place the new disclosure in context and to justify the rapid publication format. Back-to-back Letters will be considered. Full details should be reserved for an Article, which should appear in due course.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
