Comparative effects of arecoline, caffeine, and nicotine on transcription level in the nucleus accumbens of mice.

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Genomics Pub Date : 2025-01-04 DOI:10.1016/j.ygeno.2025.110986

Shaofang Huang, Xinran Wang, Feifan Zhou

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Abstract

Though widely consumed, current research on the neural mechanisms of arecoline, caffeine, and nicotine remains limited, and the similarities and differences of these substances on the nervous system are still not clear. This study used RNA-seq to analyze the gene expression in the nucleus accumbens (NAc) of mice, and compared the behavioral changes through open field and conditioned place preference (CPP), exploring the effects of different psychoactive substances at transcriptional and behavioral levels. Gene Ontology enrichment analysis revealed that nicotine and caffeine significantly alter biological processes related to synaptic function, and KEGG pathway analysis showed that the differentially expressed genes in the nicotine-treated group were significantly more enriched in pathways related to substance dependence, with arecoline showing the least enrichment. Furthermore, only acute caffeine treatment significantly increased mouse activity, and only nicotine induced CPP. These results provided a scientific basis for evaluating arecoline, caffeine, and nicotine on the nervous system.

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槟榔碱、咖啡因和尼古丁对小鼠伏隔核转录水平的影响。

槟榔碱、咖啡因和尼古丁虽然被广泛使用，但目前对其神经机制的研究仍然有限，这些物质对神经系统的异同尚不清楚。本研究利用RNA-seq技术分析小鼠伏隔核（NAc）的基因表达，并通过开放场和条件位置偏好（CPP）比较其行为变化，探讨不同精神活性物质在转录和行为水平上的影响。基因本体富集分析显示，尼古丁和咖啡因显著改变了突触功能相关的生物学过程；KEGG通路分析显示，尼古丁处理组差异表达基因在物质依赖相关通路上的富集程度显著提高，槟榔碱的富集程度最低。此外，只有急性咖啡因处理显著增加小鼠活性，只有尼古丁诱导CPP。这些结果为评价槟榔碱、咖啡因和尼古丁对神经系统的影响提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.