GDF15 promotes corneal neovascularization and RB cell progression via AKT/ERK signal pathway.

IF 1.8 4区 生物学 Q4 CELL BIOLOGY Growth factors Pub Date : 2024-10-01 Epub Date: 2025-01-07 DOI:10.1080/08977194.2024.2432948
Haijian Zheng, Wen Zheng, Shiliang Cheng, Chunguang Li, Guanglan Liu, Miao Yu, Meng Li, Xinfeng Liu, Fangfang Li, Yanluan Wan, Ling Wang
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Abstract

In this study, we aim to explore the involvement of growth differentiation factor 15 (GDF15) in both corneal neovascularization (CNV) and retinoblastoma (RB) progression. Cell migration and proliferation were assessed through Scratch assays and CCK-8 assays. Apoptosis was quantified using flow cytometry. In vitro angiogenesis was evaluated through a tube formation assay. RT-PCR and western blot analyses were employed to assess the angiogenesis-related factors. Results demonstrated that RhGDF15 has a promotional effect on the migration of RB cells. Conversely, si-GDF15 demonstrated an opposite effect. RhGDF15 exhibited an enhancement the tube formation, the levels of HIF-1α and SDF, as well as cell migration. Conversely, si-GDF15 demonstrated an opposite effect. The levels of factors were elevated by rhGDF15 in both HREC and RB cell lines. In conclusion, the downregulation of GDF15 has the potential to inhibit corneal angiogenesis and impede the migration of RB cells. These effects may be dependent on the AKT/ERK pathway.

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GDF15通过AKT/ERK信号通路促进角膜新生血管和RB细胞进展。
在这项研究中,我们旨在探讨生长分化因子15 (GDF15)在角膜新生血管(CNV)和视网膜母细胞瘤(RB)进展中的作用。通过Scratch实验和CCK-8实验评估细胞迁移和增殖。流式细胞术定量细胞凋亡。通过试管形成试验评估体外血管生成。采用RT-PCR和western blot分析血管生成相关因素。结果表明,RhGDF15对RB细胞的迁移具有促进作用。相反,si-GDF15表现出相反的效果。RhGDF15能促进小管形成、HIF-1α和SDF水平以及细胞迁移。相反,si-GDF15表现出相反的效果。在HREC和RB细胞系中,rhGDF15均升高了因子水平。综上所述,GDF15的下调可能抑制角膜血管生成,阻碍RB细胞的迁移。这些作用可能依赖于AKT/ERK通路。
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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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