Roseomonas cutis sp. nov., isolated from human epidermis.

Abstract

Five pink-pigmented bacterial strains, isolated from human skin and classified within the genus Roseomonas, were examined. Among them, four were identified as Roseomonas mucosa, while strain OT10^T was deemed to be a potential novel species. Strain OT10^T exhibited characteristics, such as Gram-stain-negative, oxidase positive, motile, strictly aerobic and rod shaped. The cells had multiple flagella at one end, arranged in a lophotrichous pattern. The predominant cellular fatty acids in OT10^T were C_18:1 ω7c/C_18:1 ω6c and C_18:1 2OH; ubiquinone (Q)-10 was identified as the sole quinone. Major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and two aminolipids. The G+C content of the genome was determined to be 72.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities revealed that strain OT10^T is closely related to Roseomonas gilardii subsp. gilardii ATCC 49956^T (97.7%), Roseomonas gilardii subsp. rosea ATCC BAA-691^T (97.7%) and R. mucosa ATCC BAA-692^T (97.5%). For the comparative genomic analyses, whole-genome sequencing was also conducted for strain OT10^T. Considering the chemotaxonomic, genotypic and phenotypic features, as well as the low average nucleotide identity and digital DNA-DNA hybridization values compared to its closest phylogenomic neighbours, OT10^T is proposed to be a novel species named Roseomonas cutis sp. nov., with OT10^T designated as the type strain (=KCTC 92087^T =JCM 34968^T).