Study on the Transformation Process of Thyroid Fine-Needle Aspiration Liquid-Based Cytology to Whole-Slide Image.

IF 1.2 4区医学 Q4 CELL BIOLOGY Cytopathology Pub Date : 2025-01-08 DOI:10.1111/cyt.13468

Yuanyuan Lei, Dongcun Wang, Yanlin Wen, Jinhui Liu, Jian Cao

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Abstract

Objective: Analyse and summarise the reasons for failure in the digital acquisition of thyroid liquid-based cytology (LBC) slides and the technical challenges, and explore methods to obtain reliable and reproducible whole digital slide images for clinical thyroid cytology.

Method: Use the glass slide scanning imaging system to acquire whole-slide image (WSI) of thyroid LBC in sdpc format through different. Statistical analysis was conducted on the different acquisition methods, the quality of the glass slides, clinical and pathological characteristics of the case, TBSRTC grading and the quality of WSI.

Results: The WSI obtained by different scanning methods showed a high level of consistency in quality (W = 0.325, p < 0.001), especially between fully automatic scanning with different focus densities (W = 0.9, p < 0.001). A total of 2114 images were obtained through different methods of multi-layer fusion and multi-point focusing scanning, with scan success rates of 100.0%, 100.0%, 100.0% and 23.6%, respectively. The correlation between the quality of thyroid LBC glass slides and the image quality of thyroid LBC WSI was statistically significant (p < 0.001). The correlation between TBSRTC grading and the quality of thyroid LBC digital WSI was statistically significant (p < 0.001).

Conclusions: Although the quality of glass slides has a significant impact, the success rate and image quality of malignant tumour scanning are both high. Overall, the risk of missed diagnosis of malignant tumours is low. In the future, we also need to improve the performance and algorithm of the scanner in cases of sparse cells.

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甲状腺细针穿刺液基细胞学到全片图像转换过程的研究。

目的：分析总结甲状腺液基细胞学（LBC）切片数字化采集失败的原因及面临的技术挑战，探索获得可靠、可复制的甲状腺细胞学全数字切片图像的方法。方法：利用玻璃载玻片扫描成像系统，通过不同的扫描方式获取sdpc格式的甲状腺LBC全片图像（WSI）。统计分析不同获取方法、玻片质量、病例临床病理特征、TBSRTC分级及WSI质量。结果：不同扫描方式所获得的WSI质量一致性较高（W = 0.325, p）。结论：虽然玻片质量对恶性肿瘤扫描的成功率和图像质量有显著影响，但其成功率和图像质量均较高。总的来说，恶性肿瘤漏诊的风险很低。在未来，我们还需要改进扫描器在稀疏单元情况下的性能和算法。

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来源期刊

Cytopathology 生物-病理学

CiteScore

2.30

自引率

15.40%

发文量

107

审稿时长

6-12 weeks

期刊介绍： The aim of Cytopathology is to publish articles relating to those aspects of cytology which will increase our knowledge and understanding of the aetiology, diagnosis and management of human disease. It contains original articles and critical reviews on all aspects of clinical cytology in its broadest sense, including: gynaecological and non-gynaecological cytology; fine needle aspiration and screening strategy. Cytopathology welcomes papers and articles on: ultrastructural, histochemical and immunocytochemical studies of the cell; quantitative cytology and DNA hybridization as applied to cytological material.