{"title":"Quantitative investigation of a 3D bubble trapper in a high shear stress microfluidic chip using computational fluid dynamics and L*A*B* color space","authors":"Warisara Boonsiri, Hein Htet Aung, Jirasin Aswakool, Siraphob Santironnarong, Phattarin Pothipan, Rungrueang Phatthanakun, Wares Chancharoen, Aekkacha Moonwiriyakit","doi":"10.1007/s10544-024-00727-w","DOIUrl":null,"url":null,"abstract":"<div><p>Microfluidic chips often face challenges related to the formation and accumulation of air bubbles, which can hinder their performance. This study investigated a bubble trapping mechanism integrated into microfluidic chip to address this issue. Microfluidic chip design includes a high shear stress section of fluid flow that can generate up to 2.7 Pa and two strategically placed bubble traps. Commercially available magnets are used for fabrication, effectively reducing production costs. The trapping efficiency is assessed through video recordings with a phone camera and analysis of captured air volumes by injecting dye at flow rates of 50, 100, and 150 µL/min. This assessment uses L*A*B* color space with analysis of the perceptual color difference ∆E and computational fluid dynamics (CFD) simulations. The results demonstrate successful application of the bubble trap mechanism for lab-on-chip bubble detection, effectively preventing bubbles from entering microchannels and mitigating potential damage. Furthermore, the correlation between the L*A*B* color space and volume fraction from CFD simulations allows accurate assessment of trap performance. Therefore, this observation leads to the hypothesis that ∆E could be used to estimate the air volume inside the bubble trap. Future research will validate the bubble trap performance in cell cultures and develop efficient methods for long-term air bubble removal.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":490,"journal":{"name":"Biomedical Microdevices","volume":"27 1","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10544-024-00727-w.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Microdevices","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10544-024-00727-w","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
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Abstract
Microfluidic chips often face challenges related to the formation and accumulation of air bubbles, which can hinder their performance. This study investigated a bubble trapping mechanism integrated into microfluidic chip to address this issue. Microfluidic chip design includes a high shear stress section of fluid flow that can generate up to 2.7 Pa and two strategically placed bubble traps. Commercially available magnets are used for fabrication, effectively reducing production costs. The trapping efficiency is assessed through video recordings with a phone camera and analysis of captured air volumes by injecting dye at flow rates of 50, 100, and 150 µL/min. This assessment uses L*A*B* color space with analysis of the perceptual color difference ∆E and computational fluid dynamics (CFD) simulations. The results demonstrate successful application of the bubble trap mechanism for lab-on-chip bubble detection, effectively preventing bubbles from entering microchannels and mitigating potential damage. Furthermore, the correlation between the L*A*B* color space and volume fraction from CFD simulations allows accurate assessment of trap performance. Therefore, this observation leads to the hypothesis that ∆E could be used to estimate the air volume inside the bubble trap. Future research will validate the bubble trap performance in cell cultures and develop efficient methods for long-term air bubble removal.
期刊介绍：
Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology is an interdisciplinary periodical devoted to all aspects of research in the medical diagnostic and therapeutic applications of Micro-Electro-Mechanical Systems (BioMEMS) and nanotechnology for medicine and biology.
General subjects of interest include the design, characterization, testing, modeling and clinical validation of microfabricated systems, and their integration on-chip and in larger functional units. The specific interests of the Journal include systems for neural stimulation and recording, bioseparation technologies such as nanofilters and electrophoretic equipment, miniaturized analytic and DNA identification systems, biosensors, and micro/nanotechnologies for cell and tissue research, tissue engineering, cell transplantation, and the controlled release of drugs and biological molecules.
Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices and nanotechnology are encouraged. A non-exhaustive list of fields of interest includes: nanoparticle synthesis, characterization, and validation of therapeutic or imaging efficacy in animal models; biocompatibility; biochemical modification of microfabricated devices, with reference to non-specific protein adsorption, and the active immobilization and patterning of proteins on micro/nanofabricated surfaces; the dynamics of fluids in micro-and-nano-fabricated channels; the electromechanical and structural response of micro/nanofabricated systems; the interactions of microdevices with cells and tissues, including biocompatibility and biodegradation studies; variations in the characteristics of the systems as a function of the micro/nanofabrication parameters.
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