How the Ectopic Expression of the Barley F-Box Gene HvFBX158 Enhances Drought Resistance in Arabidopsis thaliana.

IF 5.6 2区生物学 International Journal of Molecular Sciences Pub Date : 2025-01-02 DOI:10.3390/ijms26010342

Shuting Wen, Yicheng Chen, Xingzhe Yang, Guo Zhang, Lulu Jin, Xiaoqin Zhang, Yunxia Fang, Dawei Xue

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Abstract

In this study, the drought-responsive gene HvFBX158 from barley was transferred to Arabidopsis thaliana, and overexpression lines were obtained. The phenotypic characteristics of the transgenic plants, along with physiological indicators and transcription level changes of stress-related genes, were determined under drought treatment. Under drought stress, transgenic plants overexpressing HvFBX158 exhibited enhanced drought tolerance and longer root lengths compared to wild-type plants. Additionally, malondialdehyde and hydrogen peroxide contents were significantly lower in transgenic lines, while superoxide dismutase activity was elevated. Quantitative RT-PCR showed that the expression levels of drought and stress response genes, including AtP5CS, AtDREB2A, AtGSH1, AtHSP17.8, and AtSOD, were significantly upregulated. Transcriptome analysis further confirmed that HvFBX158 regulated multiple stress tolerance pathways. In summary, the overexpression of the HvFBX158 gene enhanced drought tolerance in Arabidopsis thaliana by regulating multiple stress response pathways. This study provides a practical basis for improving drought-resistant barley varieties and lays a foundation for subsequent research on F-box family genes for stress resistance in barley.

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大麦F-Box基因HvFBX158异位表达增强拟南芥抗旱性的机制

本研究将大麦干旱响应基因HvFBX158转移到拟南芥中，获得了过表达系。测定转基因植株在干旱条件下的表型特征、生理指标和胁迫相关基因的转录水平变化。在干旱胁迫下，与野生型植物相比，过表达HvFBX158的转基因植物表现出更强的耐旱性和更长的根长。丙二醛和过氧化氢含量显著降低，超氧化物歧化酶活性显著升高。定量RT-PCR结果显示，干旱和胁迫应答基因AtP5CS、AtDREB2A、AtGSH1、AtHSP17.8和AtSOD的表达水平显著上调。转录组分析进一步证实HvFBX158调控多种胁迫耐受途径。综上所述，HvFBX158基因的过表达通过调控多种胁迫响应途径增强了拟南芥的抗旱性。本研究为大麦抗旱品种的改良提供了实践依据，为后续大麦抗旱F-box家族基因的研究奠定了基础。

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来源期刊

International Journal of Molecular Sciences 化学-化学综合

自引率

10.70%

发文量

13472

审稿时长

1.7 months

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).