Assessment of bacteriological and immunological markers in urinary tract infection and the effect of antibiotics on the isolated bacteria.

IF 1.5 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Cellular and molecular biology Pub Date : 2025-01-12 DOI:10.14715/cmb/2024.70.12.17
Wisam F Hameed, Bashar S Noomi, Alaa A Khaleel, Dhouha Ghribi
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Abstract

Urinary tract infections (UTIs) are recognized as the second most common medical condition, following respiratory infections. Despite the availability of numerous efficacious antibiotics for the management of UTIs, the rising incidence of bacterial resistance presents significant challenges in the treatment of these infections. Bacteria are endowed with the ability to reproduce and develop resistance mechanisms against antibiotics. The current investigation aimed to evaluate the susceptibility of bacterial isolates from urinary tract infections (UTIs) to a variety of antibiotics, including ciprofloxacin, trimethoprim, amikacin, gentamicin, tetracycline, chloramphenicol, nalidixic acid, nitrofurantoin, meropenem, and novobiocin. Additionally, the study sought to quantify the levels of the inflammatory immune marker interleukin-6 (IL-6) in UTI patients. It also explored the correlation between IL-6 levels in UTI patients and healthy controls, as well as the relationship between IL-6 levels and blood parameters in both infected and healthy individuals. The present study involved the collection of 155 samples from patients diagnosed with urinary tract infections of both genders and varying age groups, ranging from 15 to 75 years, at Salah al-Din General Hospital. The findings revealed that 102 urine samples tested positive for bacterial growth, resulting in a prevalence rate of 68%. In contrast, 53 urine samples were negative for bacterial growth, reflecting a prevalence rate of 32%. The diagnostic outcomes for all isolates, following the application of laboratory diagnostic methodologies, revealed a diverse array of bacterial species, including Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus saprophyticus, Staphylococcus aureus, and Staphylococcus epidermidis. The immunological analysis revealed a statistically significant increase (p < 0.05) in IL-6 concentrations in the positive control group compared to the levels observed in the infected cohort. Our study concluded that significant antibiotic resistance in UTI pathogens, emphasizing the need for tailored treatments.

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尿路感染细菌学和免疫学指标的评估及抗生素对分离细菌的影响。
尿路感染(UTI)被认为是继呼吸道感染之后的第二大常见疾病。尽管目前有多种有效的抗生素可用于治疗尿路感染,但细菌耐药性的不断增加给这些感染的治疗带来了巨大挑战。细菌具有繁殖和发展抗生素耐药性机制的能力。本次调查旨在评估尿路感染(UTI)细菌分离物对多种抗生素的敏感性,包括环丙沙星、三甲氧苄啶、阿米卡星、庆大霉素、四环素、氯霉素、萘啶酸、硝基呋喃妥因、美罗培南和新生物素。此外,该研究还试图量化尿毒症患者的炎症免疫标志物白细胞介素-6(IL-6)的水平。研究还探讨了UTI 患者和健康对照组 IL-6 水平之间的相关性,以及感染者和健康人 IL-6 水平与血液参数之间的关系。本研究在萨拉赫丁总医院收集了 155 份尿液样本,这些样本来自 15 至 75 岁不同年龄段的尿路感染患者,其中包括男性和女性。研究结果显示,102 份尿液样本的细菌生长呈阳性,患病率为 68%。相比之下,53 份尿样的细菌生长呈阴性,患病率为 32%。应用实验室诊断方法对所有分离物进行诊断的结果显示,细菌种类繁多,包括大肠埃希菌、肺炎克雷伯菌、变形杆菌、铜绿假单胞菌、溶血性葡萄球菌、金黄色葡萄球菌和表皮葡萄球菌。免疫学分析表明,阳性对照组的 IL-6 浓度与感染组相比有显著的统计学增长(p < 0.05)。我们的研究得出结论,UTI 病原体对抗生素具有明显的耐药性,这强调了采取针对性治疗的必要性。
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来源期刊
Cellular and molecular biology
Cellular and molecular biology 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
331
期刊介绍: Cellular and Molecular Biology publishes original articles, reviews, short communications, methods, meta-analysis notes, letters to editor and comments in the interdisciplinary science of Cellular and Molecular Biology linking and integrating molecular biology, biophysics, biochemistry, enzymology, physiology and biotechnology in a dynamic cell and tissue biology environment, applied to human, animals, plants tissues as well to microbial and viral cells. The journal Cellular and Molecular Biology is therefore open to intense interdisciplinary exchanges in medical, dental, veterinary, pharmacological, botanical and biological researches for the demonstration of these multiple links.
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