A photosynthesis-derived bionic system for sustainable biosynthesis

Abstract

“Cell factory” strategy based on microbial anabolism pathways offers an intriguing alternative to relieve the dependence on fossil fuels, which are recognized as the main sources of CO₂ emission. Typically, anabolism of intracellular substance in cell factory requires the consumption of sufficient reduced nicotinamide adenine dinucleotide /nicotinamide adenine dinucleotide phosphate NAD(P)H and adenosine triphosphate ATP. However, it is of great challenge to modify the natural limited anabolism and to increase the insufficient level of NAD(P)H and ATP to optimum concentrations without causing metabolic disorder. Inspired by the natural photosynthesis process in which NAD(P)H and ATP can both be produced through the coupled electron-proton transfer processes driven by sunlight, herein we designed a light-driven bionic system composed of three modules including photo-induced electron module, electron transfer channel module and proton gradient module. The proposed strategy of light-driven bionic system enables for achieving simultaneous and controllable supplies of NAD(P)H and ATP, thus facilitating both highly efficient CO₂ fixation and biomanufacturing. The proposed light-driven bionic system design strategy in this work might pave new sustainable ways for reducing power and energy regeneration to optimize microbial metabolism, offering intriguing alternatives for CO₂ emission mitigation and high-value chemical biomanufacturing.