Protective effects of tiger milk mushroom extract (xLr®) against UVB irradiation in Caenorhabditis elegans via DAF-16 anti-oxidant regulation.

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional and Complementary Medicine Pub Date : 2024-11-12 eCollection Date: 2025-01-01 DOI:10.1016/j.jtcme.2024.11.004

Panthakarn Rangsinth, Rajasekharan Sharika, Chanin Sillapachaiyaporn, Sunita Nilkhet, Kamonwan Chaikhong, Kanika Verma, Anchalee Prasansuklab, Szu-Ting Ng, Chon-Seng Tan, Shin-Yee Fung, Tewin Tencomnao, Siriporn Chuchawankul

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Abstract

Background and aim: A critical causative factor of oxidative stress and inflammation leading to several skin complications is ultraviolet-B (UVB) irradiation. Lignosus rhinocerus (LR), or tiger milk mushroom, is native to Southeast Asia. Cold water extract of an LR cultivar, TM02® (xLr®) is a promising anti-oxidant and anti-inflammatory source. However, the effects of xLr® on UVB-induced photoaging have never been elucidated.

Experimental procedure: This study investigated the protective effects of xLr® and its high, medium, and low molecular weight (HLR, MLR, and LLR, respectively) fractions against UVB irradiation using in vivo Caenorhabditis elegans (C. elegans) model.

Results and conclusion: The investigation revealed a significant lifespan extension of xLr® and its fractions in UVB-irradiated C. elegans, which could be mediated by the regulation of genes associated with anti-oxidant (daf-16 and sod-3) and apoptosis (cep-1, hus-1, ced-13, and egl-1) pathways. xLr® significantly reduced the ROS production in C. elegans and increased the DAF-16 nuclear translocation compared to untreated worms. Additionally, the SOD-3 expression was increased in the xLr®-treated worms. Hence, it suggests that the different components in xLr® work synergistically to protect against UVB irradiation. Our findings may be beneficial for the application of xLr® as a treatment against UVB-induced cellular damage and photoaging.

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虎奶菇提取物（xLr®）通过DAF-16抗氧化调控对秀丽隐杆线虫抗UVB辐射的保护作用

背景与目的：紫外线b （UVB）照射是引起氧化应激和炎症的一个关键因素，可导致多种皮肤并发症。虎乳菇（Lignosus rhinocerus， LR），或称虎乳菇，原产于东南亚。TM02®（xLr®）是一种很有前途的抗氧化和抗炎来源。然而，xLr®对uvb诱导的光老化的影响尚未被阐明。实验步骤：本研究采用秀丽隐杆线虫（C. elegans）体内模型，研究xLr®及其高、中、低分子量组分（分别为HLR、MLR和LLR）对UVB辐射的保护作用。结果和结论：研究发现xLr®及其组分在uvb照射下的秀丽隐杆线虫中具有显著的延长寿命作用，其作用可能通过调控与抗氧化（daf-16和sod-3）和凋亡（cep-1、hus-1、ce -13和egl-1）通路相关的基因介导。与未处理的线虫相比，xLr®显著降低了线虫体内ROS的产生，增加了DAF-16核易位。此外，在xLr®处理的蠕虫中，SOD-3的表达增加。因此，这表明xLr®中的不同成分协同作用，以防止UVB辐射。我们的发现可能有助于xLr®作为一种治疗uvb诱导的细胞损伤和光老化的方法。

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来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.