In Vitro Antioxidant and Antibacterial Activities of Ethyl Acetate Extracts of Ziziphus lotus Leaves and Five Associated Endophytic Fungi.

IF 4.7 2区生物学 Q2 MICROBIOLOGY Microorganisms Pub Date : 2024-12-23 DOI:10.3390/microorganisms12122671

Amel Ghazi-Yaker, Bart Kraak, Jos Houbraken, El-Hafid Nabti, Cristina Cruz, Noria Saadoun, Karim Houali

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Abstract

The exploration of new pharmacological compounds from endophytic fungi offers infinite possibilities. The aim of this study was to evaluate the antibacterial and antioxidant activities of extracts from the leaves of Ziziphus lotus and five of its endophytic fungi and investigate the chemical diversity of the secondary metabolites produced. Isolated, purified, and molecularly identified endophytes and plant leaves were subjected to ethyl acetate extraction. The antibacterial potential of the extracts was assessed by the disc diffusion method against five bacterial strains: Staphylococcus aureus ATCC 25923; Staphylococcus aureus MU50; Enterococcus faecalis WDCM00009; Escherichia coli ATCC 25922; and Pseudomonas aeruginosa ATCC 27853. DPPH and reducing power tests were performed to assess antioxidant potential. GC-MS analysis was used to identify volatile compounds in extracts. Fungal endophytes were identified as Aspergillus cavernicola, Aspergillus persii, Alternaria alternata, Cladosporium asperlatum, and Fusarium incarnatum-equiseti complex, with respective accession numbers DTO 412-G6, DTO 412-I5, DTO 413-E7, DTO 412-G4, and DTO 414-I2. GC-MS analysis revealed a large number of bioactive compounds. All extracts showed antibacterial activity against at least two of the bacteria tested, and most showed antioxidant activity. The Aspergillus cavernicola extract stood out for its higher phenolic content and higher antioxidant and antibacterial activities in all tests.

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酸枣叶乙酸乙酯提取物及5种伴生内生真菌的体外抗氧化和抗菌活性研究。

从内生真菌中探索新的药理化合物提供了无限的可能性。本研究旨在评价酸枣叶及其5种内生真菌提取物的抑菌和抗氧化活性，并研究其次生代谢产物的化学多样性。分离、纯化和分子鉴定内生菌和植物叶片进行乙酸乙酯萃取。采用圆盘扩散法对5株金黄色葡萄球菌ATCC 25923；金黄色葡萄球菌MU50；粪肠球菌WDCM00009；大肠杆菌ATCC 25922；铜绿假单胞菌ATCC 27853。通过DPPH和还原力试验来评价其抗氧化能力。采用气相色谱-质谱法对提取物中的挥发性成分进行鉴定。真菌内生真菌鉴定为海绵曲霉、巴西曲霉、互交霉、曲霉枝孢菌和牛毛镰刀菌复合体，登记号分别为DTO 412-G6、DTO 412-I5、DTO 413-E7、DTO 412-G4和DTO 414-I2。GC-MS分析显示了大量的生物活性化合物。所有提取物都对至少两种细菌具有抗菌活性，并且大多数提取物都具有抗氧化活性。海绵曲霉提取物具有较高的酚类物质含量和抗氧化、抗菌活性。

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来源期刊

Microorganisms Medicine-Microbiology (medical)

CiteScore

7.40

自引率

6.70%

发文量

2168

审稿时长

20.03 days

期刊介绍： Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.