Chemical biology research in RIKEN NPDepo aimed at agricultural applications.

IF 4.4 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Proceedings of the Japan Academy. Series B, Physical and Biological Sciences Pub Date : 2025-01-01 DOI:10.2183/pjab.101.003

Hiroyuki Osada

{"title":"Chemical biology research in RIKEN NPDepo aimed at agricultural applications.","authors":"Hiroyuki Osada","doi":"10.2183/pjab.101.003","DOIUrl":null,"url":null,"abstract":"<p><p>This review outlines research on chemical biology using mainly microbial metabolites for agricultural applications. We established the RIKEN Natural Products Depository (NPDepo), housing many microbial metabolites, to support academic researchers who focus on drug discovery. We studied methods to stimulate secondary metabolism in microorganisms to collect various microbial products. The switch of secondary metabolism in microorganisms changes depending on the culture conditions. We discovered compounds that activate biosynthetic gene clusters in actinomycetes and filamentous fungi. Using these compounds, we succeeded in inducing the production of active compounds. Two approaches for screening bioactive compounds are described. One is phenotypic screening to explore antifungal compounds assisted by artificial intelligence (AI). AI can distinguish the morphological changes induced by antifungal compounds in filamentous fungi. The other is the chemical array method for detecting interactions between compounds and target proteins. Our chemical biology approach yielded many new compounds as fungicide candidates.</p>","PeriodicalId":20707,"journal":{"name":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","volume":"101 1","pages":"8-31"},"PeriodicalIF":4.4000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Japan Academy. Series B, Physical and Biological Sciences","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.2183/pjab.101.003","RegionNum":3,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

This review outlines research on chemical biology using mainly microbial metabolites for agricultural applications. We established the RIKEN Natural Products Depository (NPDepo), housing many microbial metabolites, to support academic researchers who focus on drug discovery. We studied methods to stimulate secondary metabolism in microorganisms to collect various microbial products. The switch of secondary metabolism in microorganisms changes depending on the culture conditions. We discovered compounds that activate biosynthetic gene clusters in actinomycetes and filamentous fungi. Using these compounds, we succeeded in inducing the production of active compounds. Two approaches for screening bioactive compounds are described. One is phenotypic screening to explore antifungal compounds assisted by artificial intelligence (AI). AI can distinguish the morphological changes induced by antifungal compounds in filamentous fungi. The other is the chemical array method for detecting interactions between compounds and target proteins. Our chemical biology approach yielded many new compounds as fungicide candidates.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

理化学研究所 NPDepo 以农业应用为目标的化学生物学研究。

本文综述了主要利用微生物代谢物进行农业应用的化学生物学研究。我们建立了RIKEN天然产物库（NPDepo），储存了许多微生物代谢物，以支持专注于药物发现的学术研究人员。我们研究了刺激微生物次生代谢的方法，以收集各种微生物产物。微生物次生代谢的开关随培养条件的不同而变化。我们在放线菌和丝状真菌中发现了激活生物合成基因簇的化合物。利用这些化合物，我们成功地诱导了活性化合物的产生。介绍了筛选生物活性化合物的两种方法。一是人工智能（AI）辅助下的表型筛选，探索抗真菌化合物。人工智能可以区分丝状真菌抗真菌化合物引起的形态变化。另一种是用于检测化合物与靶蛋白之间相互作用的化学阵列方法。我们的化学生物学方法产生了许多新的化合物作为候选的杀菌剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences 生物-综合性期刊

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Proceedings of the Japan Academy Ser. B (PJA-B) is a scientific publication of the Japan Academy with a 90-year history, and covers all branches of natural sciences, except for mathematics, which is covered by the PJA-A. It is published ten times a year and is distributed widely throughout the world and can be read and obtained free of charge through the world wide web.