Lucia Mamede, Gabriel W Rangel, Lahngong Methodius Shinyuy, Naïma Boussif, Marie-France Herent, Bernadette Govaerts, Olivia Jansen, Allison Ledoux, Pascal De Tullio, Joëlle Quetin-Leclercq, Manuel Llinás, Michel Frédérich
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引用次数: 0
Abstract
Background: Artemisia spp. have been used for millennia in traditional medicine to treat a variety of ailments, including malaria. Extracts of Artemisia afra and A. annua remain widely used throughout Africa for healthcare purposes, notably to prevent and/or treat malaria. However, the modes of action of these plant extracts remain unclear, with contradictory reports regarding the presence and role of artemisinin in both plants.
Purpose: The aim of this study was to identify differences in the antimalarial mode of action of A. afra and A. annua by measuring their phenolic profiles and comparing their effect on parasite metabolism in vitro.
Methods: In this work, we analyzed the phenolic profile of A. afra and A. annua extracts through high-performance liquid chromatography (HPLC), detected and quantified artemisinin through HPLC and mass spectrometry (MS), and performed comparative HPLC-MS metabolomic analysis on in vitro-cultured Plasmodium falciparum trophozoites to elucidate the potential modes of action of these plant extracts.
Results: A. afra contained only trace amounts of artemisinin and elicited a different parasite metabolic response compared to A. annua, which contained significantly more artemisinin and correlated closely with the parasite response profile elicited by purified artemisinin. A. annua impacted parasite glutathione metabolism in agreement with the established redox activity of artemisinin, while A. afra had an effect on lipid precursors.
Conclusions: This study reveals that A. afra and A. annua have divergent effects on Plasmodium falciparum metabolism and provides support for ongoing efforts exploring the use of A. afra for the treatment of malaria.
背景：千百年来，传统医学一直使用蒿属植物来治疗包括疟疾在内的各种疾病。在整个非洲，黄花蒿和青蒿的提取物仍被广泛用于保健目的，特别是预防和/或治疗疟疾。目的：本研究的目的是通过测量 A. afra 和 A. annua 的酚类物质含量并比较其对寄生虫体外代谢的影响，来确定这两种植物提取物抗疟作用模式的差异：在这项工作中，我们通过高效液相色谱法（HPLC）分析了 A. afra 和 A. annua 提取物的酚类概况，通过 HPLC 和质谱法（MS）检测并量化了青蒿素，并对体外培养的恶性疟原虫滋养体进行了 HPLC-MS 代谢组学比较分析，以阐明这些植物提取物的潜在作用模式：A. afra仅含有微量的青蒿素，与A. annua相比，A. annua含有更多的青蒿素，与纯化青蒿素引起的寄生虫反应特征密切相关，但A. afra引起的寄生虫代谢反应与A. annua不同。青蒿素影响寄生虫的谷胱甘肽代谢，这与青蒿素已确定的氧化还原活性一致，而Afra则对脂质前体有影响：这项研究揭示了 A. afra 和 A. annua 对恶性疟原虫新陈代谢的不同影响，并为目前探索使用 A. afra 治疗疟疾的努力提供了支持。
期刊介绍：
Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
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