Determination of the Content of FAD Cofactor and NAD(P)H-Oxidase Complexes in Mouse Splenocytes and Lewis Carcinoma Cells Under Conditions of Apoptosis by Confocal Microscopy Method
E. M. Mil, A. A. Albantova, L. I. Matienko, A. N. Goloshchapov, M. A. Korovin, V. V. Kuvyrkova
{"title":"Determination of the Content of FAD Cofactor and NAD(P)H-Oxidase Complexes in Mouse Splenocytes and Lewis Carcinoma Cells Under Conditions of Apoptosis by Confocal Microscopy Method","authors":"E. M. Mil, A. A. Albantova, L. I. Matienko, A. N. Goloshchapov, M. A. Korovin, V. V. Kuvyrkova","doi":"10.1134/S1990793124701227","DOIUrl":null,"url":null,"abstract":"<p>In this paper, using fluorescence and confocal microscopy, we study the content of the flavin adenine nucleotide (FAD) cofactor and enzymatic NAD(P)H-oxidase complexes (with fluorophores Annexin V‑ FITC, 7-AAD (7-aminoactinomycin D), EtBr) under conditions of apoptosis caused by sodium anphen with hydrogen peroxide in healthy mouse splenocytes and Lewis carcinoma tumor cells. The use of fluorescence microscopy allows observing and quantifying the apoptotic effect of sodium anphen and hydrogen peroxide, and it also allows visualizing the metabolic changes in the cell, including the increased fluorescence of FAD in tumor cells and NAD(P)H-oxidase complexes in splenocytes. The data obtained indicate the possibility of using sodium anphen in combination with hydrogen peroxide as an antitumor drug acting on certain types of cells.</p>","PeriodicalId":768,"journal":{"name":"Russian Journal of Physical Chemistry B","volume":"18 6","pages":"1560 - 1565"},"PeriodicalIF":1.4000,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Physical Chemistry B","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1134/S1990793124701227","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSICS, ATOMIC, MOLECULAR & CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
In this paper, using fluorescence and confocal microscopy, we study the content of the flavin adenine nucleotide (FAD) cofactor and enzymatic NAD(P)H-oxidase complexes (with fluorophores Annexin V‑ FITC, 7-AAD (7-aminoactinomycin D), EtBr) under conditions of apoptosis caused by sodium anphen with hydrogen peroxide in healthy mouse splenocytes and Lewis carcinoma tumor cells. The use of fluorescence microscopy allows observing and quantifying the apoptotic effect of sodium anphen and hydrogen peroxide, and it also allows visualizing the metabolic changes in the cell, including the increased fluorescence of FAD in tumor cells and NAD(P)H-oxidase complexes in splenocytes. The data obtained indicate the possibility of using sodium anphen in combination with hydrogen peroxide as an antitumor drug acting on certain types of cells.
共聚焦显微镜法测定凋亡条件下小鼠脾细胞和Lewis癌细胞中FAD辅助因子和NAD(P) h -氧化酶复合物含量
本文采用荧光共聚焦显微镜技术，研究了在苯酚钠与过氧化氢作用下健康小鼠脾细胞和Lewis癌肿瘤细胞凋亡的情况下，黄素腺嘌呤核苷酸（FAD）辅因子和酶促NAD(P) h -氧化酶复合物（含荧光团Annexin V - FITC、7-AAD（7-氨基放射线霉素D）、EtBr）的含量。使用荧光显微镜可以观察和量化苯酚钠和过氧化氢的凋亡作用，还可以观察细胞内的代谢变化，包括肿瘤细胞中FAD的荧光增加和脾细胞中NAD(P) h -氧化酶复合物的荧光。所获得的数据表明，使用苯酚钠与过氧化氢联合作为抗肿瘤药物作用于某些类型的细胞的可能性。
期刊介绍：
Russian Journal of Physical Chemistry B: Focus on Physics is a journal that publishes studies in the following areas: elementary physical and chemical processes; structure of chemical compounds, reactivity, effect of external field and environment on chemical transformations; molecular dynamics and molecular organization; dynamics and kinetics of photoand radiation-induced processes; mechanism of chemical reactions in gas and condensed phases and at interfaces; chain and thermal processes of ignition, combustion and detonation in gases, two-phase and condensed systems; shock waves; new physical methods of examining chemical reactions; and biological processes in chemical physics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
