Characteristics and spatiotemporal changes in phenotypes and genotypes of extended-spectrum β-lactamases in Escherichia coli isolated from bloodstream infections in China from 2014 to 2021.

IF 4.6 2区医学 Q1 MICROBIOLOGY Annals of Clinical Microbiology and Antimicrobials Pub Date : 2025-01-18 DOI:10.1186/s12941-025-00774-y

Sayyed Salman, Hao Xu, Yunbo Chen, Jinru Ji, Zhiying Liu, Yonghong Xiao

{"title":"Characteristics and spatiotemporal changes in phenotypes and genotypes of extended-spectrum β-lactamases in Escherichia coli isolated from bloodstream infections in China from 2014 to 2021.","authors":"Sayyed Salman, Hao Xu, Yunbo Chen, Jinru Ji, Zhiying Liu, Yonghong Xiao","doi":"10.1186/s12941-025-00774-y","DOIUrl":null,"url":null,"abstract":"Objective: To examine the characteristics and spatiotemporal changes in the phenotypes and genotypes of extended-spectrum β-lactamases (ESBLs) in Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 and 2021.Methods: 983 ESBL-positive E. coli strains were collected from BSIs in 66 hospitals across different geographic regions in China from 2014 to 2021. The phenotypic confirmation of ESBL was performed through the double-disc diffusion method. The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing.Results: Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. Among 983 phenotypically confirmed ESBL-positive E. coli, 763 (77.6%) were confirmed to carry ESBL genes, with the majority being of the CTX-M type, which is further divided into 23 subtypes and dominated by the CTX-M-9 and CTX-M-1 groups, with 457/763 and 333/763, respectively. Other ESBLs and ampC genes, such as blaOXA-1, blaCMY, and blaDHA-1, often coexisted with either the CTX-M-9 or CTX-M-1 groups. blaCTX-M-14 (34.3%, 157/457) and blaCTX-M-55 (45.9%, 153/333) were the dominant subtypes in the CTX-M-9 and CTX-M-1 groups, respectively. A notable increase in blaCTX-M-27 was observed, particularly from 2019 to 2021, with 26.4%, 23.1%, and 25.8% in all genotypes. Regarding the geographical distribution of the ESBLs, the highest rate of ESBL genetic positivity was observed in Southwest China, accounting for 84.9% (45/53), and the lowest was observed in Northeast China, with 73.2% (30/41). The abundance of the blaCTX-M-27 genotype, in particular, exhibited a notable increase in Southwest China, with 31.4% (14/45) of the strains exhibiting this genotype, followed by the CTX-M-55 genotype, with 13.6% (6/45) of the strains exhibiting this genotype.Conclusions: This study demonstrated a steadily decreasing trend in the incidence of ESBLs and predominant CTX-M type ESBLs, particularly the CTX-M-9 and CTX-M-1 groups, in E. coli strains across China, a notable increase in the blaCTX-M-27 genotype and regional variations in the ESBL gene distribution were detected.","PeriodicalId":8052,"journal":{"name":"Annals of Clinical Microbiology and Antimicrobials","volume":"24 1","pages":"7"},"PeriodicalIF":4.6000,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743024/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of Clinical Microbiology and Antimicrobials","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12941-025-00774-y","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To examine the characteristics and spatiotemporal changes in the phenotypes and genotypes of extended-spectrum β-lactamases (ESBLs) in Escherichia coli strains isolated from bloodstream infections (BSIs) across China between 2014 and 2021.

Methods: 983 ESBL-positive E. coli strains were collected from BSIs in 66 hospitals across different geographic regions in China from 2014 to 2021. The phenotypic confirmation of ESBL was performed through the double-disc diffusion method. The genetic type was determined using polymerase chain reaction (PCR) followed by DNA sequencing.

Results: Between 2014 and 2021, the prevalence of ESBL-positive E. coli steadily decreased from 61.2 to 49.6%. Among 983 phenotypically confirmed ESBL-positive E. coli, 763 (77.6%) were confirmed to carry ESBL genes, with the majority being of the CTX-M type, which is further divided into 23 subtypes and dominated by the CTX-M-9 and CTX-M-1 groups, with 457/763 and 333/763, respectively. Other ESBLs and ampC genes, such as bla_OXA-1, bla_CMY, and bla_DHA-1, often coexisted with either the CTX-M-9 or CTX-M-1 groups. bla_CTX-M-14 (34.3%, 157/457) and bla_CTX-M-55 (45.9%, 153/333) were the dominant subtypes in the CTX-M-9 and CTX-M-1 groups, respectively. A notable increase in bla_CTX-M-27 was observed, particularly from 2019 to 2021, with 26.4%, 23.1%, and 25.8% in all genotypes. Regarding the geographical distribution of the ESBLs, the highest rate of ESBL genetic positivity was observed in Southwest China, accounting for 84.9% (45/53), and the lowest was observed in Northeast China, with 73.2% (30/41). The abundance of the bla_CTX-M-27 genotype, in particular, exhibited a notable increase in Southwest China, with 31.4% (14/45) of the strains exhibiting this genotype, followed by the CTX-M-55 genotype, with 13.6% (6/45) of the strains exhibiting this genotype.

Conclusions: This study demonstrated a steadily decreasing trend in the incidence of ESBLs and predominant CTX-M type ESBLs, particularly the CTX-M-9 and CTX-M-1 groups, in E. coli strains across China, a notable increase in the bla_CTX-M-27 genotype and regional variations in the ESBL gene distribution were detected.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

2014 - 2021年中国血液感染大肠埃希菌延伸谱β-内酰胺酶表型和基因型特征及时空变化

目的：研究2014 - 2021年中国血液感染（bsi）分离大肠埃希菌扩展谱β-内酰胺酶（ESBLs）表型和基因型的特征及时空变化。方法：2014 - 2021年，在中国不同地理区域66家医院的bsi中采集983株esbl阳性大肠杆菌。通过双盘扩散法对ESBL进行表型确认。采用聚合酶链反应（PCR）确定遗传类型，然后进行DNA测序。结果：2014 - 2021年间，esbl阳性大肠杆菌的患病率从61.2%稳步下降至49.6%。在983例表型证实的ESBL阳性大肠杆菌中，763例（77.6%）被证实携带ESBL基因，其中以CTX-M型居多。CTX-M型又分为23个亚型，以CTX-M-9和CTX-M-1组为主，分别为457/763和333/763。其他ESBLs和ampC基因，如blaOXA-1、blaCMY和blaDHA-1，通常与CTX-M-9或CTX-M-1组共存。CTX-M-9组和CTX-M-1组的优势亚型分别为blaCTX-M-14（34.3%, 157/457）和blaCTX-M-55（45.9%, 153/333）。观察到blaCTX-M-27的显著增加，特别是从2019年到2021年，在所有基因型中分别为26.4%、23.1%和25.8%。从ESBL的地理分布来看，西南地区ESBL遗传阳性率最高，为84.9%(45/53)，东北地区最低，为73.2%（30/41）。其中，西南地区blaCTX-M-27基因型的丰度显著增加，为31.4%(14/45)，其次是CTX-M-55基因型，为13.6%（6/45）。结论：本研究表明，在中国大肠杆菌菌株中，ESBLs和主要的CTX-M型ESBLs，特别是CTX-M-9和CTX-M-1型ESBLs的发病率呈稳步下降趋势，blaCTX-M-27基因型显著增加，ESBL基因分布存在区域差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Annals of Clinical Microbiology and Antimicrobials MICROBIOLOGY-

CiteScore

8.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.