The protective effects of active ingredients from acrorus tatarinowii on sperm and their molecular mechanisms.

IF 2.4 3区医学 Q2 ANDROLOGY Basic and Clinical Andrology Pub Date : 2025-01-20 DOI:10.1186/s12610-024-00247-w

Zonglin Lu, Haiyang Zhao, Hui Wang, Xin Wang, Zixue Sun

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Abstract

Purpose: To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway.

Methods: Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii were identified and their target genes associated with sperm-related diseases were elucidated.Subsequently, an oligoasthenozoospermia mouse model was induced and treated with Acrorus tatarinowii. Serum hormone levels were assessed by ELISA, testicular histopathology by HE staining, and target gene expression by qPCR and Western blotting.

Results: Acrorus tatarinowii treatment significantly upregulated BCL2 expression in the testes of oligoasthenozoospermic rat. This was accompanied by improved histopathological features in testicular tissues, reduced LH and FSH levels in serum.

Conclusion: Acrorus tatarinowii exerts therapeutic effects in oligoasthenozoospermia by regulating the BCL2/Bax/Caspase3 pathway, maybe by inhibiting apoptosis, and promoting germ cell proliferation. These findings highlight its potential as a natural remedy for male infertility associated with sperm function disorders.

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石竹有效成分对精子的保护作用及其分子机制。

目的：通过调节BCL2/Bax/Caspase3信号通路，探讨羊尾蛇对少弱精子症的治疗潜力及其相关机制。方法：首先利用TCMSP和Disgenet数据库，鉴定了石首蛇的有效成分，并对其与精子相关疾病相关的靶基因进行了分析。随后，建立少弱精子症小鼠模型，并给予大鼠青霉治疗。ELISA检测血清激素水平，HE染色检测睾丸组织病理学，qPCR和Western blotting检测靶基因表达。结果：石蒜处理显著上调少精大鼠睾丸中BCL2的表达。这伴随着睾丸组织的组织病理学特征的改善，血清中LH和FSH水平的降低。结论：油梨通过调控BCL2/Bax/Caspase3通路，抑制细胞凋亡，促进生殖细胞增殖，对少弱精子症有治疗作用。这些发现强调了它作为一种自然疗法治疗与精子功能障碍相关的男性不育症的潜力。

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来源期刊

Basic and Clinical Andrology Medicine-Urology

CiteScore

3.50

自引率

0.00%

发文量

审稿时长

22 weeks

期刊介绍： Basic and Clinical Andrology is an open access journal in the domain of andrology covering all aspects of male reproductive and sexual health in both human and animal models. The journal aims to bring to light the various clinical advancements and research developments in andrology from the international community.